Mouse Anti-Rat GluN2A/NR2A Monoclonal IgG2b, Clone S327A-38
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Catalog numberSMC-434D-BI
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PricePlease ask
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Size100 µg
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CloneS327A-38
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ImmunogenFusion protein amino acids 75-325 (extracellular N-terminus) of rat GluN2A/NR2A
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Antibody s full descriptionMouse Anti-Rat NR2A Monoclonal IgG2b Antibody, Clone: S327A-38: Biotin
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameNMDA 2A Antibody, NMDAR2A Antibody, NMDAR 2A Antibody, NMDA Receptor 2A Antibody, Glutamate Receptor Antibody, GRIN2A Antibody, Glutamate [NMDA] Receptor subunit epsilon-1 Antibody, Glutamate receptor ionotropic N methyl D aspartate 2A Antibody, HNR2A Antibody, N methyl D aspartate receptor channel Antibody, subunit epsilon 1 Antibody, N Methyl D Aspartate Receptor Subtype 2A Antibody, N methyl D aspartate receptor subunit 2A Antibody, NMDA receptor subtype 2A Antibody, NMDA Receptor Type 2A Antibody, OTTHUMP00000160135 Antibody, OTTHUMP00000174531 Antibody
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Raised inMouse
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Antibody s targetNR2A
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Primary research fieldsNeuroscience, Neurotransmitter Receptors, Glutamate Receptors, NMDA Receptors, Cell Signaling
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~170kDa. Does not react with NR2B.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-434 was sufficient for detection of GluN2A/NR2A in 20 µg of COS cells transiently transfected with GFP-tagged NR2A lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane , Cell Junction
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextN-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D).
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Bibliography1. Teng H.J., et al. (2010) PLoS ONE. 5: e13342.
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Released date1-Mar-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_036705.3
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Gene number24409
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Protein numberQ00959
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327A-38 (SMC-434). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody (SMC-434) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-GluN2A/NR2A Antibody. (C) Composite. Western Blot analysis of Monkey COS cells transfected with GFP-tagged NR2A showing detection of ~170 kDa GluN2A/NR2A protein using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327A-38 (SMC-434). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NR2A. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody (SMC-434) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa. Mouse Anti-GluN2A/NR2A Antibody [S327A-38] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-434) Mouse Anti-GluN2A/NR2A Antibody [S327A-38] used in Western Blot (WB) on Monkey COS cells transfected with GFP-tagged NR2A (SMC-434)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolIGLVI-38, SNORD115-38, IGHV3-38, IGKV2D-38, IGKV2-38, IGHVIII-38-1, RNU6-38P, TPPP3, IGHV4-38-2, IGHV1-38-4
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Short nameMouse Anti- GluN2A/NR2A Monoclonal IgG2b, Clone S327A-38
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2b, IgG2b
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LabelBiotin
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat GluN2A/NR2A monoclonal IgG2b, clonality S327A-38
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Alternative techniquemurine, antibodies
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Clone nameS327A-38
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable (I)-38 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable (I)-38
- immunoglobulin lambda variable (I)-38 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-38
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-38 (non-functional)
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Synonyms gene name
- immunoglobulin heavy variable 3-38
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2D-38 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2D-38
- immunoglobulin kappa variable 2D-38 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2-38 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2-38
- immunoglobulin kappa variable 2-38 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-38-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-38-1
- immunoglobulin heavy variable (III)-38-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 38, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 38
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Locus
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Discovery year2008-06-10
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene nametubulin polymerization promoting protein family member 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-05-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Tubulin polymerization promoting proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 4-38-2
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 4-B (provisional)
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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Classification
- Immunoglobulin heavy locus at 14q32.33
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 1-38-4 (non-functional)
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 1-C (provisional)
- immunoglobulin heavy variable 1-C (provisional, non-functional)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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Classification
- Immunoglobulin heavy locus at 14q32.33
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
