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Clone
7B8
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Immunogen
Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638)
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Antibody s full description
Mouse Anti-Rat ENaC beta Monoclonal IgG1 Antibody, Clone: 7B8: ATTO 680
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
SCNN1B Antibody, Amiloride sensitive sodium channel subunit beta Antibody, Amiloride-sensitive sodium channel beta-subunit Antibody, Beta ENaC Antibody, Beta NaCH Antibody, ENaC beta Antibody, ENaCB Antibody, Epithelial Na(+) channel subunit beta Antibody, Epithelial Na+ channel beta subunit Antibody, Epithelial Na+ channel subunit beta Antibody, Epithelial sodium channel beta 2 subunit Antibody, Epithelial sodium channel beta 3 subunit Antibody, Nonvoltage gated sodium channel 1 beta subunit Antibody, Nonvoltage gated sodium channel 1 subunit beta Antibody, Nonvoltage-gated sodium channel 1 beta subunit Antibody, SCNEB Antibody, SCNN 1B Antibody, Sodium channel nonvoltage gated 1 beta (Liddle syndrome) Antibody, Sodium channel nonvoltage gated 1 beta Antibody
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Raised in
Mouse
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Antibody s target
ENaC beta
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Primary research fields
Neuroscience, Ion Channels, Sodium Channels, Epithelial Sodium Channels (ENaC)
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Brandname
None
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Antibodies applications
WB, IHC
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Antibody s reactivity
Mouse
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Antibody s dilutions
WB (1:1000); IHC (1:150); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~87kDa.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
A 1:1000 dilution of SMC-240 was sufficient for detection of ENaC beta in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Apical Cell Membrane
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
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Bibliography
1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.
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Released date
12-Sep-2016
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_036780
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Gene number
24767
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Protein number
P37090
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8 (SMC-240). Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody (SMC-240) at 1:150. Localization: Collecting duct principal cells. Magnification: 60X. Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~87kDa ENaC beta protein using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8 (SMC-240). Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 µg. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody (SMC-240) at 1:1000. Predicted/Observed Size: ~87kDa. Mouse Anti-ENaC beta Antibody [7B8] used in Immunohistochemistry (IHC) on Mouse Kidney (cortex) (SMC-240) Mouse Anti-ENaC beta Antibody [7B8] used in Western Blot (WB) on Mouse Whole kidney homogenates (SMC-240)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
