Mouse Anti-Human VAChT Monoclonal IgG1, Clone S6-38
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Catalog numberSMC-393D-A655
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PricePlease ask
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Size100 µg
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CloneS6-38
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ImmunogenSynthetic peptide amino acids 521-532 of human VAChT
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Antibody s full descriptionMouse Anti-Human VAChT Monoclonal IgG1 Antibody, Clone: S6-38: ATTO 655
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameVesicular Acetylcholine Transporter Antibody, MGC12716 Antibody, rVAT Antibody, Slc18a3 Antibody, Solute carrier family 18 (vesicular acetylcholine) member 3 Antibody, Solute carrier family 18 (vesicular monoamine) member 3 Antibody, Solute carrier family 18 member 3 Antibody
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Raised inMouse
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Antibody s targetVAChT
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Primary research fieldsNeuroscience, Cell Structure, Pre-Synaptic Markers, Pumps/Transporters, Neurotransmitter Transporters
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:200), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~56kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA dilution of 1:50-1:200 of SMC-341 was sufficient for detection of VAChT Transporter in rat brain using immunohistochemistry analysis and goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextVAChT is a member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicle for release into the extracellular space. Acetylcholine (Ach) transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
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Bibliography1. Erickson J.D., Varoqui H. (2000) FASEB J. 14(15): 2450-2458. 2. Weihe E., Tao-Cheng J.H., Schafer M.K., Erickson J.D., Eiden L.E. (1996) Proc Natl Acad Sci USA. 93(8): 3547-3552.
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Released date16-May-2012
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_003046.2
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Gene number6572
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Protein numberQ16572
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38 (SMC-393). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody (SMC-393) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) VAChT Antibody (D) Composite. Western Blot analysis of Rat brain membrane lysate showing detection of VAChT protein using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38 (SMC-393). Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody (SMC-393) at 1:1000. Mouse Anti-VAChT Antibody [S6-38] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-393) Mouse Anti-VAChT Antibody [S6-38] used in Western Blot (WB) on Rat brain membrane lysate (SMC-393)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolRPS6, IGLVI-38, SNORD115-38, IGHV3-38, IGKV2D-38, IGKV2-38, IGHVIII-38-1, MRPS6, RNU6-38P, TPPP3
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Short nameMouse Anti- VAChT Monoclonal IgG1, Clone S6-38
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 655
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman VAChT monoclonal IgG1, clonality S6-38
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Alternative techniquemurine, antibodies
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Clone nameS6-38
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Gene info
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Identity
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Gene
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Long gene nameribosomal protein S6
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Synonyms
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Synonyms name
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Locus
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Discovery year1990-07-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- S ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable (I)-38 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable (I)-38
- immunoglobulin lambda variable (I)-38 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-38
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-38 (non-functional)
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Synonyms gene name
- immunoglobulin heavy variable 3-38
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2D-38 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2D-38
- immunoglobulin kappa variable 2D-38 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2-38 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2-38
- immunoglobulin kappa variable 2-38 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-38-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-38-1
- immunoglobulin heavy variable (III)-38-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemitochondrial ribosomal protein S6
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Synonyms gene
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Synonyms gene name
- chromosome 21 open reading frame 101
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-02-28
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Entrez gene record
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RefSeq identity
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Classification
- Mitochondrial ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 38, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 38
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Locus
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Discovery year2008-06-10
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene nametubulin polymerization promoting protein family member 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-05-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Tubulin polymerization promoting proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data