Mouse Anti-Human TrpC7 Monoclonal IgG1, Clone S64A-36
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Catalog numberSMC-343D-A700
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PricePlease ask
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Size100 µg
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CloneS64A-36
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ImmunogenSynthetic peptide amino acids 845-862 of human TRPC7
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Antibody s full descriptionMouse Anti-Human TRPC7 Monoclonal IgG1 Antibody, Clone: S64A-36: ATTO 700
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameTRP7 Antibody, KNP3 Antibody, TRPM2 Antibody, transient receptor potential cation channel subfamily C member 7 Antibody
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Raised inMouse
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Antibody s targetTRPC7
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Primary research fieldsNeuroscience, Ion Channels, Transient Receptor Potential Channels, Cell Signaling
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~100kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-343 was sufficient for detection of TrpC7 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextTransient receptor potential cation channel, subfamily C, member 7, also known as TRPC7, is a non-selective cation channel that is directly activated by DAG. TrpC7 shows constitutive activity and susceptibility to negative regulation by extracellular Ca2+. Because of this, TrpC7 plays an important role in the Ca2+ signaling pathway (1). TrpC7 is also expressed abundantly in the heart, and combined with its ability to act as a Ca2+ channel, TrpC7 might contribute to the process of heart failure (2).
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Bibliography1. Numaga T., Wakamori M., and Mori Y. (2007) Handb Exp Pharmacol. 179: 143-151. 2. Satoh S., et al. (2007) Mol Cell Biochem. 294(1-2): 205-215.
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Released date31-Mar-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_065122.1
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Gene number57113
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Protein numberQ9HCX4
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone S64A-36 (SMC-343). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (SMC-343) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything. Western Blot analysis of Rat brain membrane lysate showing detection of TrpC7 protein using Mouse Anti-TrpC7 Monoclonal Antibody, Clone S64A-36 (SMC-343). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (SMC-343) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone S64A-36 (SMC-343). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (SMC-343) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear staining . Mouse Anti-TrpC7 Antibody [S64A-36] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-343) Mouse Anti-TrpC7 Antibody [S64A-36] used in Western Blot (WB) on Rat brain membrane lysate (SMC-343) Mouse Anti-TrpC7 Antibody [S64A-36] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-343)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolSNORD115-36, IGKV2D-36, IGHV3-36, IGKV2-36, IGLV1-36, SNORA35, RNU6-36P, PDLIM1, THAP4, ZNF608
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Short nameMouse Anti- TrpC7 Monoclonal IgG1, Clone S64A-36
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 700
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman TrpC7 monoclonal IgG1, clonality S64A-36
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Alternative techniquemurine, antibodies
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Clone nameS64A-36
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-36
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2D-36 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2D-36
- immunoglobulin kappa variable 2D-36 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-36 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-36
- immunoglobulin heavy variable 3-36 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2-36 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2-36
- immunoglobulin kappa variable 2-36 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 1-36
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, H/ACA box 35
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Synonyms
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Locus
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Discovery year2006-04-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, H/ACA box
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 36, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 36
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Locus
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Discovery year2008-06-10
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene namePDZ and LIM domain 1
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Synonyms gene
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Synonyms gene name
- PDZ and LIM domain 1 (elfin)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-01-21
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Entrez gene record
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Pubmed identfication
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Classification
- LIM domain containing
- PDZ domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTHAP domain containing 4
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-10-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- THAP domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namezinc finger protein 608
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-03-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data