Mouse Anti-Human PINK1 Monoclonal IgG1, Clone S4-15

  • Clone
    S4-15
  • Immunogen
    Fusion protein amino acids 112-496 (cytoplasmic C-terminus) of human PINK1. 82% identical to rat and 81% identical to mouse. >30% identity with DMPK.
  • Antibody s full description
    Mouse Anti-Human PINK1 Monoclonal IgG1 Antibody, Clone: S4-15: FITC
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Parkinson disease (autosomal recessive) 6 Antibody, EC 2.7.11.1 Antibody, PARK6 Antibody, serine/threonine-protein kinase PINK1 mitochondrial Antibody, PTEN-induced putative kinase protein 1 Antibody, protein kinase BRPK Antibody, BRPK Antibody, FLJ27236 Antibody, PTEN induced putative kinase 1 Antibody, Phosphatase and Tensin Homolog Antibody
  • Raised in
    Mouse
  • Antibody s target
    PINK1
  • Primary research fields
    Neuroscience, Cell Signaling, Post-translational Modifications, Phosphorylation, cardiovascular System, Heart
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH 7.4, 50% glycerol, 0.1% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~50kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-450 was sufficient for detection of PINK1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Mitochondrion , Mitochondrion Outer Membrane , Cytoplasm
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 is synthesized as a 63 kD protein which undergoes proteolyt processing to generate at least two cleaved forms (55 kD and 42 kD). PINK1 and its substrates have been found in the cytosol as well as in different sub-mitochondrial compartments, and according to the recent reports; PINK1 may be targeted to OMM (outer mitochondrial membrane) with its kinase domain facing the cytosol, providing a possible explanation for the observed physical interaction with the cytosolic E3 ubiquitin ligase Parkin. Defective PINK1 may cause alterations in processing, stability, localization and activity as well as binding to substrates/interaction-partners which ultimately leads to differential effects on mitochondrial function and morphology. Mutations in PINK1 are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of alpha-synuclein, as well as proteasome dysfunction.
  • Released date
    26-Sep-2013
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_115785.1
  • Gene number
    65018
  • Protein number
    Q9BXM7.1
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PINK1 Monoclonal Antibody, Clone S4-15 (SMC-450). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody (SMC-450) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PINK1 Antibody (D) Composite. Western Blot analysis of Rat Brain showing detection of ~50 kDa PINK1 protein using Mouse Anti-PINK1 Monoclonal Antibody, Clone S4-15 (SMC-450). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody (SMC-450) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa. Mouse Anti-PINK1 Antibody [S4-15] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-450) Mouse Anti-PINK1 Antibody [S4-15] used in Western Blot (WB) on Rat Brain (SMC-450)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    PINK1-AS, PINK1, PIRC67, PIRC66, PIRC71, PIRC75, PIRC68, PIRC62, PIRC72, PSMC1
  • Short name
    Mouse Anti- PINK1 Monoclonal IgG1, Clone S4-15
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    FITC
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse Antibody toHuman phosphatase and tensin homolog induced putative kinase 1 monoclonal IgG1, clonality S4-15
  • Alternative technique
    murine, antibodies
  • Alternative to gene target
    PTEN induced putative kinase 1, PINK1 and IDBG-92922 and ENSG00000158828 and 65018, transferase activity, nuclei, Pink1 and IDBG-199773 and ENSMUSG00000028756 and 68943, PINK1 and IDBG-645657 and ENSBTAG00000006084 and 510683
  • Clone name
    S4-15
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 67
  • Locus
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 66
  • Locus
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 71
  • Locus
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 75
  • Locus
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 68
  • Locus
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 62
  • Locus
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 72
  • Locus
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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Mouse Anti PINK1 Monoclonal IgG1 Clone S4 15 - Gentaur.com
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