Mouse Anti-Human PHYH/PAHX Monoclonal IgG1, Clone S210-5
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Catalog numberSMC-472D-BI
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PricePlease ask
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Size100 µg
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CloneS210-5
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ImmunogenFusion protein amino acids 1-338 (full-length) of human PhyH. Rat: 78% identity (264/338 amino acids identical). Mouse: 78% identity (264/338 amino acids identical).
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Antibody s full descriptionMouse Anti-Human PHYH Monoclonal IgG1 Antibody, Clone: S210-5: Biotin
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Antibody s categoryMonoclonal Antibodies
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Antibody s other namePhytanoyl-CoA dioxygenase Antibody, peroxisomal Antibody, PAHX Antibody, peroxisomal Antibody, PhyH Antibody, PHYH1 Antibody, Phytanic acid oxidase Antibody, phytanoil-CoA alpha hydroxylase Antibody, phytanoyl CoA 2 hydroxylase Antibody, Phytanoyl CoA 2 oxoglutarate dioxygenase Antibody, Phytanoyl CoA alpha hydroxylase Antibody, Phytanoyl CoA dioxygenase Antibody, Phytanoyl CoA dioxygenase peroxisomal Antibody, Phytanoyl-CoA alpha-hydroxylase Antibody, Phytanoyl-CoA dioxygenase Antibody, RD Antibody, LN1 Antibody, LNAP1 Antibody, OTTHUMP00000019131 Antibody, OTTHUMP00000019132 Antibody, OTTHUMP00000179083 Antibody, OTTHUMP00000216226, Antibody
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Raised inMouse
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Antibody s targetPHYH
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Primary research fieldsNeuroscience, Cell Signaling, Organelle Markers, Trafficking
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BrandnameNone
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Antibodies applicationsWB, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~40kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-472 was sufficient for detection of PHYH/PAHX in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellPeroxisome
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextPhytanoyl-CoA alpha-hydroxylase (PHYH) is also called Phytanoyl-CoA dioxygenase, peroxisomal, Phytanic acid oxidase and the gene names PHYH or PAHX. PAHX is involved in lipid metabolism and converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. PAHX is expressed in liver, kidney, and T-cells. Defects in PHYH can cause Refsum disease (RD).
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Released date20-Nov-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_001032626.1
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Gene number5264
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Protein numberO14832
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-PHYH/PAHX Monoclonal Antibody, Clone S210-5 (SMC-472). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PHYH/PAHX Monoclonal Antibody (SMC-472) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Peroxisome, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PHYH/PAHX Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of ~40 kDa PHYH/PAHX protein using Mouse Anti-PHYH/PAHX Monoclonal Antibody, Clone S210-5 (SMC-472). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-PHYH/PAHX Monoclonal Antibody (SMC-472) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~40 kDa. Mouse Anti-PHYH/PAHX Antibody [S210-5] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-472) Mouse Anti-PHYH/PAHX Antibody [S210-5] used in Western Blot (WB) on Rat Brain Membrane (SMC-472)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolPHYH, PHYHIP, MIR1302-5, PIRC23, PIRC22, PIRC20, PIRC21, HTR6, IGHV2OR16-5, IGKV3OR2-5
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Short nameMouse Anti- PHYH/PAHX Monoclonal IgG1, Clone S210-5
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelBiotin
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman PHYH/PAHX monoclonal IgG1, clonality S210-5
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Alternative techniquemurine, antibodies
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Clone nameS210-5
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Gene info
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Identity
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Gene
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Long gene namephytanoyl-CoA 2-hydroxylase
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Synonyms gene name
- phytanoyl-CoA hydroxylase (Refsum disease)
- phytanoyl-CoA hydroxylase
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Synonyms
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Synonyms name
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Locus
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Discovery year1997-10-27
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namephytanoyl-CoA 2-hydroxylase interacting protein
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Synonyms gene
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Synonyms gene name
- phytanoyl-CoA hydroxylase interacting protein
- DYRK1A interacting protein 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-11-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-5
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 23
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 22
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 20
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 21
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene name5-hydroxytryptamine receptor 6
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Synonyms gene name
- 5-hydroxytryptamine (serotonin) receptor 6
- 5-hydroxytryptamine (serotonin) receptor 6, G protein-coupled
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-12-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 5-hydroxytryptamine receptors, G protein-coupled
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 2/OR16-5 (non-functional)
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Synonyms gene name
- immunoglobulin heavy variable 2/OR16-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 3/OR2-5 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 3/OR2-5
- immunoglobulin kappa variable 3/OR2-5 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
