Mouse Anti-Human PEX6 Monoclonal IgG1, Clone S233-8
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Catalog numberSMC-470D-A565
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PricePlease ask
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Size100 µg
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CloneS233-8
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ImmunogenFusion protein amino acids 2-294 (N-terminus) of human PEX6. Rat: 82% identity (243/293 amino acids identical). Mouse: 82% identity (244/294 amino acids identical).
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Antibody s full descriptionMouse Anti-Human PEX6 Monoclonal IgG1 Antibody, Clone: S233-8: ATTO 565
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Antibody s categoryMonoclonal Antibodies
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Antibody s other namePAF 2 Antibody, Peroxin 6 Antibody, Peroxisomal AAA type ATPase 1 Antibody, Peroxisomal biogenesis factor 6 Antibody, PXAAA1 Antibody, peroxisomal-type ATPase 1 Antibody, peroxisome biogenesis factor 6 Antibody
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Raised inMouse
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Antibody s targetPEX6
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Primary research fieldsNeuroscience, Cell Signaling, Trafficking, Cell Signaling, Organelle Markers
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BrandnameNone
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Antibodies applicationsWB, ICC/IF
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Antibody s reactivityHuman, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~100kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-470 was sufficient for detection of PEX6 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm , Peroxisome , Peroxisome Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThis gene encodes a member of the AAA (ATPases associated with diverse cellular activities) family of ATPases. This member is a predominantly cytoplasmic protein, which plays a direct role in peroxisomal protein import and is required for PTS1 (peroxisomal targeting signal 1, a C-terminal tripeptide of the sequence ser-lys-leu) receptor activity. Mutations in this gene cause peroxisome biogenesis disorders of complementation group 4 and complementation group 6.
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Released date26-Sep-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_000278.3
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Gene number5190
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Protein numberQ13608
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-PEX6 Monoclonal Antibody, Clone S233-8 (SMC-470). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PEX6 Monoclonal Antibody (SMC-470) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Peroxisome, Peroxisome Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PEX6 Antibody (D) Composite. Western Blot analysis of Rat Brain showing detection of ~100 kDa PEX6 protein using Mouse Anti-PEX6 Monoclonal Antibody, Clone S233-8 (SMC-470). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-PEX6 Monoclonal Antibody (SMC-470) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa. Mouse Anti-PEX6 Antibody [S233-8] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-470) Mouse Anti-PEX6 Antibody [S233-8] used in Western Blot (WB) on Rat Brain (SMC-470)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolPEX6, SNORD114-8, RNU6-8, SNORD113-8, SNORD116-8, IGKV2OR2-8, MIR1302-8, PIRC35
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Short nameMouse Anti- PEX6 Monoclonal IgG1, Clone S233-8
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 565
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman PEX6 monoclonal IgG1, clonality S233-8
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Alternative techniquemurine, antibodies
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Clone nameS233-8
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Gene info
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Identity
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Gene
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Long gene nameperoxisomal biogenesis factor 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-05-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Peroxins
- AAA ATPases
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-8
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 8
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 113-8
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Synonyms
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Locus
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Discovery year2006-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-8
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2/OR2-8 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2/OR2-8
- immunoglobulin kappa variable 2/OR2-8 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa (IGK) orphons
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-8
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 35
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data