Mouse Anti-Human PARP Monoclonal IgG1, Clone M3
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Catalog numberSMC-259D
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PricePlease ask
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Size100 µl
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CloneM3
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ImmunogenA synthesized peptide derived from human PARP
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Antibody s full descriptionMouse Anti-Human PARP Monoclonal IgG1 Antibody, Clone: M3: Unconjugated
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody, ADP ribosyltransferase diphtheria toxin like 1 antibody, ADP ribosyltransferase NAD(+) antibody, ADPRT 1 antibody, ADPRT antibody, ADPRT1 antibody, ARTD1 antibody, msPARP antibody, NAD(+) ADP ribosyltransferase 1 antibody, NAD(+) ADP-ribosyltransferase 1 antibody, pADPRT 1 antibody, pADPRT1 antibody, PARP 1 antibody, PARP antibody, PARP-1 antibody, PARP1 antibody, PARP1_HUMAN antibody, Poly (ADP ribose) polymerase 1 antibody, poly (ADP ribose) polymerase family, member 1 antibody, Poly [ADP-ribose] polymerase 1 antibody, Poly(ADP ribose) polymerase antibody, poly(ADP ribose) synthetase antibody, poly(ADP ribosyl)transferase antibody, Poly[ADP ribose] synthetase 1 antibody, Poly[ADP-ribose] synthase 1 antibody, PPOL antibody
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Raised inMouse
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Antibody s targetPARP
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BrandnameNone
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Antibodies applicationsWB
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Antibody s reactivityHuman
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Antibody s dilutionsWB (1:2000); optimal dilutions for assays should be determined by the user.
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PurityPeptide Affinity Purified
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Antibody buffer for storagePBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
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Antibody s specificityAntibody can detects endogenous PARP protein.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA 1:2000 dilution of SMC-259 was sufficient for detection of PARP in 10 µg of Human HeLa, 293T, Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
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Antibody in cellNucleus
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextPoly (ADP-ribose) polymerase (PARP) is a family of proteins involved in a number of cellular processes involving mainly DNA repair and programmed cell death. It is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
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Bibliography1. Piskunova TS. (2008) Curr Gerontol Geriatr Res: 754190. 2. Espinoza LA. et al. (2007) Free Radic. Biol. Med. 42 (9): 1430–40. 3. Zerfaoui M. et al. (2008) Cell. Signal. 20 (1): 186–94.
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Released date06-Sep-2016
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_001609.2
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Gene number142
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Protein numberP09874
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Antibody s datasheetContact our support service
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Representative figure legendWestern Blot analysis of Human HeLa, 293T, Jurkat cell lysates showing detection of ~113 kDa PARP protein using Mouse Anti-PARP Monoclonal Antibody, Clone M3 (SMC-259). Lane 1: Human HeLa. Lane 2: Human 293T. Lane 3: Human Jurkat.. Primary Antibody: Mouse Anti-PARP Monoclonal Antibody (SMC-259) at 1:2000. Predicted/Observed Size: ~113 kDa. Mouse Anti-PARP Antibody [M3] used in Western Blot (WB) on Human HeLa, 293T, Jurkat cell lysates (SMC-259)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolTNKS2, TIPARP, PARP1, PARP12, TNKS
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Short nameMouse Anti- PARP Monoclonal IgG1, Clone M3
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelUnconjugated
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman PARP monoclonal IgG1, clonality M3
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Alternative techniquemurine, antibodies
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Clone nameM3
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Gene info
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Identity
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Gene
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Long gene nametankyrase 2
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Synonyms gene name
- tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-05-10
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Entrez gene record
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
- Sterile alpha motif domain containing
- Ankyrin repeat domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTCDD inducible poly(ADP-ribose) polymerase
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Synonyms gene name
- TCDD-inducible poly(ADP-ribose) polymerase
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-12-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepoly(ADP-ribose) polymerase 1
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Synonyms gene
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Synonyms gene name
- ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
- poly (ADP-ribose) polymerase family, member 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers PARP-type
- Zinc fingers
- Poly(ADP-ribose) polymerases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepoly(ADP-ribose) polymerase family member 12
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Synonyms gene
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Synonyms gene name
- zinc finger CCCH-type domain containing 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-12-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
- Zinc fingers CCCH-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametankyrase
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Synonyms gene name
- tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-01-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
- Sterile alpha motif domain containing
- Ankyrin repeat domain containing
- MicroRNA protein coding host genes
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data