Mouse Anti-Human Malin Monoclonal IgG1, Clone S85-18
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Catalog numberSMC-444D-P594
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PricePlease ask
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Size100 µg
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CloneS85-18
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ImmunogenFusion protein amino acids 2-125 (N-terminus encompassing RING domain) of human Malin. 86% identical to rat, and 77% identical to mouse.
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Antibody s full descriptionMouse Anti-Human Malin Monoclonal IgG1 Antibody, Clone: S85-18: PE/ATTO 594
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameE3 ubiquitin-protein ligase NHLRC1 Antibody, NHLRC 1 Antibody, NHL repeat containing 1 Antibody, EPM2A Antibody, EPM2B Antibody, MGC119262 Antibody, MGC119264 Antibody, MGC119265 Antibody, NHL repeat containing protein 1 Antibody
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Raised inMouse
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Antibody s targetMalin
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Primary research fieldsNeuroscience, Cell Signaling, Post-translational Modifications, Ubiquitination
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BrandnameNone
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Antibodies applicationsWB, ICC/IF
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Antibody s reactivityHuman
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~42kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-444 was sufficient for detection of malin in 20 µg of transiently (malin) transfected COS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellEndoplasmic Reticulum , Nucleus
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextProgressive myoclonic epilepsy type 2 (EPM2), also called Lafora disease, is an autosomal recessive disease characterized by grand mal seizures and/or myoclonus at about 15 years of age. Rapid and severe mental deterioration follows, often with psychotic features. Survival is less than 10 years after onset. Starch-like, endoplasmic reticulum-associated polyglucosans, called Lafora bodies, can be observed in brain, muscle, liver and heart. One cause of Lafora disease is due to mutations in NHLRC1, the gene encoding Malin. Forty-nine different mutations in NHLRC1 have been shown to cause EPM2. Malin, also called NHL repeat-containing protein 1, is a single subunit E3 ubiquitin ligase, containing 6 NHL repeats and 1 RING-type zinc finger. Malin’s RING domain is responsible for its ability to mediate ubiquitination. Malin interacts with and polyubiquitinates Laforin, a protein also implicated in EPM2. Malin localizes to the endoplasmic reticulum and, to a lesser extent, in the nucleus. Malin is expressed in brain, cerebellum, spinal cord, medulla, heart, liver, skeletal muscle and pancreas.
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Bibliography1. Chan E.M, et al. (2003) Nat. Genet. 35:125-127. 2. Worby C.A, et al. (2008) J. Biol. Chem. 283:4069-4076. 3. Gomez-Abad C, et al. (2005) Neurology. 64:982-986.
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Released date11-Dec-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_940988.2
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Gene number378884
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Protein numberQ6VVB1
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 (SMC-444). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody (SMC-444) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Malin Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of ~42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 (SMC-444). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with flag-tagged Malin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody (SMC-444) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42 kDa. Mouse Anti-Malin Antibody [S85-18] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-444) Mouse Anti-Malin Antibody [S85-18] used in Western Blot (WB) on Monkey COS cells transfected with flag-tagged Malin (SMC-444)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolSNORD114-18, PIRC96, SNORD116-18, IGLV2-18, IGHV1-18, SNORD115-18, ERVW-18, IGKV2D-18, IGKV2-18
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Short nameMouse Anti- Malin Monoclonal IgG1, Clone S85-18
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelPE/ATTO 594
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman Malin monoclonal IgG1, clonality S85-18
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Alternative techniquemurine, antibodies
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Clone nameS85-18
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-18
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 96
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-18
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 2-18
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 1-18
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-18
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group W member 18
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Synonyms gene name
- endogenous retrovirus group W, member 18
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GenBank acession
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Locus
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Discovery year2013-10-21
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2D-18 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2D-18
- immunoglobulin kappa variable 2D-18 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2-18 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2-18
- immunoglobulin kappa variable 2-18 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
