Mouse Anti-Human Laforin Monoclonal IgG1, Clone S84-37
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Catalog numberSMC-466D-APC
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PricePlease ask
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Size100 µg
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CloneS84-37
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ImmunogenFusion protein amino acids 1-331 (full-length) of human Laforin. Rat: 90% identity (296/327 amino acids identical). Mouse: 89% identity (295/327 amino acids identical)
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Antibody s full descriptionMouse Anti-Human Laforin Monoclonal IgG1 Antibody, Clone: S84-37: APC
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameEPM2 Antibody, Epilepsy progressive myoclonus type 2 Lafora disease (laforin) Antibody, Epilepsy progressive myoclonus type 2A Lafora disease (laforin) Antibody, EPM2 Antibody, Epm2a Antibody, Lafora PTPase Antibody, LAFPTPase Antibody, LD Antibody, LDE Antibody, MELF Antibody
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Raised inMouse
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Antibody s targetLaforin
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Primary research fieldsNeuroscience, Cell Signaling, Post-translational Modifications, Phosphorylation
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BrandnameNone
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Antibodies applicationsWB, ICC/IF
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Antibody s reactivityHuman
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~40kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-466 was sufficient for detection of Laforin in 20 µg of human brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellEndoplasmic Reticulum
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextLaforin, also known as Lafora PTPase, is a dual specificity protein phosphatase. Laforin is involved in the control of glycogen metabolism, specifically in preventing the formation of poorly branched glycogen molecules (polyglucosans). Laforin forms a complex with NHLRC1/malin and HSP70 that suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Laforin is expressed in heart, skeletal muscle, kidney, pancreas and brain. Defective Laforin is linked to progressive myoclonic epilepsy type 2 (EPM2).
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Released date29-Oct-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_001018051.1
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Gene number7957
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Protein numberO95278
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Laforin Monoclonal Antibody, Clone S84-37 (SMC-466). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Laforin Monoclonal Antibody (SMC-466) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Laforin Antibody (D) Composite. Western Blot analysis of Human Hippocampus showing detection of ~40 kDa and 45 kDa Laforin protein using Mouse Anti-Laforin Monoclonal Antibody, Clone S84-37 (SMC-466). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Human Hippocampus. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Laforin Monoclonal Antibody (SMC-466) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~40 kDa and 45 kDa. Mouse Anti-Laforin Antibody [S84-37] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-466) Mouse Anti-Laforin Antibody [S84-37] used in Western Blot (WB) on Human Hippocampus (SMC-466)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolSNORD115-37, IGLV5-37, IGHV3-37, IGKV1-37, IGKV1D-37, RNU6-37P, LIN37, RNVU1-14, NIP7, ZC3H14
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Short nameMouse Anti- Laforin Monoclonal IgG1, Clone S84-37
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelAPC
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman Laforin monoclonal IgG1, clonality S84-37
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Alternative techniquemurine, antibodies
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Clone nameS84-37
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-37
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 5-37
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-37 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-37
- immunoglobulin heavy variable 3-37 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1-37 (non-functional)
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Synonyms gene name
- immunoglobulin kappa variable 1-37
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-37 (non-functional)
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Synonyms gene name
- immunoglobulin kappa variable 1D-37
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 37, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 37
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Locus
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Discovery year2008-06-10
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene namelin-37 DREAM MuvB core complex component
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Synonyms gene name
- lin-37 homolog (C. elegans)
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-05-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, variant U1 small nuclear 14
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Synonyms
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Locus
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Discovery year2013-04-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Variant U1 small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namenucleolar pre-rRNA processing protein NIP7
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Synonyms gene name
- nuclear import 7 homolog (S. cerevisiae)
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-12-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ribosomal biogenesis factors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namezinc finger CCCH-type containing 14
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-05-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers CCCH-type
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data