Mouse Anti-Human KCNQ4 Monoclonal IgG1, Clone S43-6
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Catalog numberSMC-309D-HRP
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PricePlease ask
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Size100 µg
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CloneS43-6
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ImmunogenFusion protein amino acids 2-77 of human KCNQ4
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Antibody s full descriptionMouse Anti-Human KCNQ4 Monoclonal IgG1 Antibody, Clone: S43-6: HRP
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameDFNA 2 antibody, DFNA2 antibody, KCNQ 4 antibody, Kcnq4 antibody, KCNQ4_HUMAN antibody, KQT like 4 antibody, KQT-like 4 antibody, KV7.4 antibody, Potassium channel KQT like 4 antibody, Potassium channel subunit alpha KvLQT4 antibody, Potassium voltage gated channel KQT like protein 4 antibody, Potassium voltage gated channel KQT like subfamily member 4 antibody, Potassium voltage gated channel subfamily KQT member 4 antibody, Potassium voltage-gated channel subfamily KQT member 4 antibody, Voltage gated potassium channel subunit Kv7.4 antibody, Voltage-gated potassium channel subunit Kv7.4 antibody
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Raised inMouse
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Antibody s targetKCNQ4
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Primary research fieldsNeuroscience, Ion Channels, Potassium Channels, Voltage-Gated Potassium Channels, Cancer
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~77kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-309 was sufficient for detection of KCNQ4 in 10 µg of COS-1 cell lysate transiently expressing KCNQ4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellPlasma Membrane , Basal Cell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe protein encoded by this gene forms a potassium channel that is thought to play a critical role in the regulation of neuronal excitability (1), particularly in sensory cells of the cochlea (2). The current generated by this channel is inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug (3).
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Bibliography1. Hernandez C.C., Zaiko O., Tolstykh G.P., Shapiro M.S. (2008) J Physiol. 586(7): 1811-1821. 2. Kharkovets T., et al. (2006) EMBO J. 25(3): 642-652. 3. Tatulian L., Delmas P., Abogadie F.C., Brown D.A. (2001) J Neuroscience. 21(15): 5535-5545.
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Released date30-Jul-2009
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_004691.2
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Gene number9132
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Protein numberP56696
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone S43-6 (SMC-309). Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody (SMC-309) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Western Blot analysis of Rat tissue lysate showing detection of KCNQ4 protein using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone S43-6 (SMC-309). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody (SMC-309) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone S43-6 (SMC-309). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody (SMC-309) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Mouse Anti-KCNQ4 antibody [S43-6] used in Immunohistochemistry (IHC) on mouse frozen brain section (SMC-309) Mouse Anti-KCNQ4 Antibody [S43-6] used in Western Blot (WB) on Rat tissue lysate (SMC-309) Mouse Anti-KCNQ4 Antibody [S43-6] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-309)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolKCNQ4, MIR1302-6, PIRC28, PIRC24, PIRC26, PIRC27, PIRC25, ADCP1
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Short nameMouse Anti- KCNQ4 Monoclonal IgG1, Clone S43-6
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelHRP
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman KCNQ4 monoclonal IgG1, clonality S43-6
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Alternative techniquemurine, antibodies
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Clone nameS43-6
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Gene info
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Identity
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Gene
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Long gene namepotassium voltage-gated channel subfamily Q member 4
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Synonyms gene
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Synonyms gene name
- potassium voltage-gated channel, KQT-like subfamily, member 4
- potassium channel, voltage gated KQT-like subfamily Q, member 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-02-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Potassium voltage-gated channels
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-6
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 28
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 24
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 26
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 27
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 25
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene nameadenosine deaminase complexing protein 1
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data