Mouse Anti-Human HSP70/HSC70 Monoclonal IgG, Clone N27F3-4
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Catalog numberSMC-104A
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Price:
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Size50 µg
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CloneN27F3-4
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ImmunogenRecombinant HSP70/HSC70
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Antibody s full descriptionMouse Anti-Human HSP70/HSC70 Monoclonal IgG Antibody, Clone: N27F3-4: Unconjugated
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameHSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
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Raised inMouse
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Antibody s targetHSP70/HSC70
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Primary research fieldsCancer, Heat Shock
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP, FCM, IEM
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Antibody s reactivityHuman, Mouse, Rat, Bovine, Nematode (Caenorhabditis elegans), Beluga, Dog, Chicken, Fruit Fly (Drosophila melanogaster), Fish, Guinea Pig (Cavia porcellus), Hamster, Monkey, Pig, Plant, Cucumber, Pea (Pisum sativum), Rabbit, Sheep, African clawed frog (Xenopus laevis)
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Antibody s dilutionsWB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.2, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~72 (HSP) and ~73kDa (HSC).
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-104 was sufficient for detection of HSP70/HSC70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextHSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at more.
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Bibliography1. Welch W.J. and Suhan J.P. (1986) J.Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993), J. Mol. Evol.38 (1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990), Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Polanonka-Grabowska R. et. al. (1997) Blood 90: 1516-1526. 8. Schnell D.J. et. al. (1994) Science 266: 1007-1012. 9. Kabakov A.E., et. al. (2002) Am. J.Physiol. 283(2): C521-C534. 10. Ricart J. et. al. (1997) Biochem. J. 324: 635-643. 11. Hang H. and Fox M.H. (1995) Cytometry 19(2): 119-125.
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Released date1-Jan-2007
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PubMed number24447452, 24447452, 23958398, 22831866, 22504172, 22194796, 21445704, 20435172
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Tested applicationsWestern Blot, Immunohistochemistry, Immunohistochemistry, Western Blot, Western Blot, Western Blot, Immunocytochemistry/Immunofluorescence, Western Blot
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Tested reactivityHuman, Human, Rat, Human, Human, Rat, Hamster, Human
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NCBI numberNP_005336.3
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Gene number3303
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Protein numberP08107
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 (SMC-104). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-104) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis. Western Blot analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 (SMC-104). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-104) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Hsp70 Antibody [N27] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-104) Mouse Anti-Hsp70 Antibody [N27] used in Western Blot (WB) on Human Cell lysates (SMC-104)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.05
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolHSPA14, HSPA1B, HSPA1A, HSPA1L, HSPA8, MIR1302-4, PIRC18, PIRC16
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Short nameMouse Anti- HSP70/HSC70 Monoclonal IgG, Clone N27F3-4
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Techniqueanti-Human, anti-, Mouse, anti, IgG, antibody to, antibodies, antibodies against human proteins, IgGs, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG, IgG
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LabelUnconjugated
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman HSP70/HSC70 monoclonal Immunoglobulin G, clonality N27F3-4
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Alternative techniquemurine, antibodies, immunoglobulins
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Clone nameN27F3-4
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Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 14
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Synonyms gene name
- heat shock 70kDa protein 14
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-04-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Heat shock 70kDa proteins
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 1B
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Synonyms gene name
- heat shock 70kD protein 1B
- heat shock 70kDa protein 1B
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Synonyms
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Locus
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Discovery year1995-08-11
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Entrez gene record
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Pubmed identfication
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Classification
- TIM23 complex
- Heat shock 70kDa proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 1A
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Synonyms gene
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Synonyms gene name
- heat shock 70kD protein 1A
- heat shock 70kDa protein 1A
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Classification
- TIM23 complex
- Heat shock 70kDa proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 1 like
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Synonyms gene name
- heat shock 70kD protein-like 1
- heat shock 70kDa protein 1-like
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-05-12
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Entrez gene record
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Pubmed identfication
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Classification
- TIM23 complex
- Heat shock 70kDa proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 8
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Synonyms gene
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Synonyms gene name
- heat shock 70kD protein 8
- heat shock 70kDa protein 8
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-05-08
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Entrez gene record
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Pubmed identfication
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Classification
- Spliceosomal P complex
- Heat shock 70kDa proteins
- Spliceosomal C complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-4
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 18
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 16
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data