Mouse Anti-Human HSP70 Monoclonal IgM, Clone 2A4
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Catalog numberSMC-163D-A700
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Price:
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Size100 µg
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Clone2A4
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ImmunogenHuman recombinant HSP70 overexpressed in E.coli
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Antibody s full descriptionMouse Anti-Human HSP70 Monoclonal IgM Antibody, Clone: 2A4: ATTO 700
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameHSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSP73 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
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Raised inMouse
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Antibody s targetHSP70
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Primary research fieldsCancer, Heat Shock
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP, AM
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Antibody s reactivityHuman, Mouse, Rat, Amphibians, Chicken, Fish, Yeast, Yeast (Saccharomyces cerevisiae), Fruit Fly (Drosophila melanogaster)
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Antibody s dilutionsWB (1:5000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityPEG Purified
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Antibody buffer for storagePBS pH7.2, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~70kDa. May detect HSP70, HSC70, and HSP72.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis0.2 µg/ml of SMC-163 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgM:HRP as the secondary antibody.
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Antibody in cellCytoplasm
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextHSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at more.
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Bibliography1. Balashova N. et al. (2005) J Biol Chem 280:2186-96. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nat Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.
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Released date1-Nov-2008
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_005336.3
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Gene number3303
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Protein numberP08107
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 2A4 (SMC-163). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (SMC-163) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X. Western Blot analysis of Rat Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 2A4 (SMC-163). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-163) at 1:5000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-HSP70 Antibody [2A4] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical Cancer cell line (HeLa) (SMC-163) Mouse Anti-Hsp70 Antibody [2A4] used in Western Blot (WB) on Rat Cell lysates (SMC-163)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolHSPA1B, HSPA1A, HSPA14, HSPA1L
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Short nameMouse Anti- HSP70 Monoclonal IgM, Clone 2A4
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Techniqueanti-Human, anti-, Mouse, anti, IgM, antibody to, antibodies, antibodies against human proteins, IgMs, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgM, IgM
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LabelATTO 700
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman HSP70 monoclonal Immunoglobulin M, clonality 2A4
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Alternative techniquemurine, antibodies, immunoglobulin-m
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Clone name2A4
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Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 1B
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Synonyms gene name
- heat shock 70kD protein 1B
- heat shock 70kDa protein 1B
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Synonyms
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Locus
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Discovery year1995-08-11
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Entrez gene record
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Pubmed identfication
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Classification
- TIM23 complex
- Heat shock 70kDa proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 1A
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Synonyms gene
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Synonyms gene name
- heat shock 70kD protein 1A
- heat shock 70kDa protein 1A
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Classification
- TIM23 complex
- Heat shock 70kDa proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 14
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Synonyms gene name
- heat shock 70kDa protein 14
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-04-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Heat shock 70kDa proteins
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family A (Hsp70) member 1 like
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Synonyms gene name
- heat shock 70kD protein-like 1
- heat shock 70kDa protein 1-like
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-05-12
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Entrez gene record
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Pubmed identfication
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Classification
- TIM23 complex
- Heat shock 70kDa proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data