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Clone
1F2-H5
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Immunogen
Full length human HSC70
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Antibody s full description
Mouse Anti-Human HSC70 (HSP73) Monoclonal IgG2a Kappa Antibody, Clone: 1F2-H5: ATTO 488
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
HSC54 Antibody, HSC71 Antibody, HSC73 Antibody, HSP71 Antibody, HSP73 Antibody, HSPA10 Antibody, HSPA8 Antibody, LAP1 Antibody, NIP71 Antibody
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Raised in
Mouse
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Antibody s target
HSC70 (HSP73)
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Primary research fields
Cancer, Heat Shock
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF, IP, ELISA, PLA, PBA, AM
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~73kDa. Does not cross react with HSP70.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-151 was sufficient for detection of HSC70 in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm , Melanosome
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).
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Bibliography
1. Brown C.L. et al. (1993) J.Cell Biol., 120 (5): 1101-1112. 2. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993)J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989), Cell 59: 591-601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C., and Valencia A. (1992) Proc. Nut1Acad. Sci. USA 89: 7290-7294. 6. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-840.
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Released date
7-Jan-2015
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PubMed number
28053100, 25552722, 25552722, 23942053, 23942053, 23091061, 21811788
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Tested applications
Western Blot, Western Blot, Immunocytochemistry/Immunofluorescence, Western Blot, Immunoprecipitation , ELISA, Protein Binding Assay
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Tested reactivity
Human, Human, Human, Rat, Rat, Human, Rat
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NCBI number
NP_006588.1
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Gene number
3312
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Protein number
P11142
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5 (SMC-151). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody (SMC-151) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite. Western Blot analysis of Human Cell lysates showing detection of Hsc70 protein using Mouse Anti-Hsc70 Monoclonal Antibody, Clone 1F2-H5 (SMC-151). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsc70 Monoclonal Antibody (SMC-151) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5 (SMC-151). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody (SMC-151) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsc70 Monoclonal Antibody, Clone 1F2-H5 (SMC-151). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Hsc70 Monoclonal Antibody (SMC-151) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Bright cytoplasmic staining, duller nuclear staining. Mouse Anti-Hsc70 (Hsp73) Antibody [1F2-H5] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-151) Mouse Anti-Hsc70 Antibody [1F2-H5] used in Western Blot (WB) on Human Cell lysates (SMC-151) Mouse Anti-Hsc70 (Hsp73) Antibody [1F2-H5] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-151) Mouse Anti-Hsc70 Antibody [1F2-H5] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-151)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.2
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
