Mouse Anti-Human GFAP R416WT Monoclonal IgG1, Clone S206B-9
-
Catalog numberSMC-442D-A488
-
PricePlease ask
-
Size100 µg
-
-
CloneS206B-9
-
ImmunogenSynthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP; 100% identical in rat and mouse. >50% identity with other proteins (Vimentin, Desmin and Peripherin).
-
Antibody s full descriptionMouse Anti-Human GFAP Monoclonal IgG1 Antibody, Clone: S206B-9: ATTO 488
-
Antibody s categoryMonoclonal Antibodies
-
Antibody s other nameGlial fibrillary acidic protein Antibody, Intermediate filament protein Antibody, Astrocyte Antibody, gfapl Antibody, DKFZp459C0729 Antibody, MGC139638 Antibody, FLJ45472 Antibody, AI836096 Antibody, GFAP Antibody
-
Raised inMouse
-
Antibody s targetGFAP
-
Primary research fieldsNeuroscience, Cell Structure, Astrocyte Markers
-
BrandnameNone
-
Antibodies applicationsWB, IHC, ICC/IF
-
Antibody s reactivityHuman, Mouse, Rat
-
Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
-
PurityProtein G Purified
-
Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
-
Antibody s concentration1 mg/ml
-
Antibody s specificityDetects ~50kDa. Does not cross-react with GFAP-R416W or other proteins (based on KO validation results).
-
Storage recommendations-20ºC
-
Shipping recommendationsBlue Ice or 4ºC
-
Antibody certificate of analysis1 µg/ml of SMC-442 was sufficient for detection of GFAP R416WT in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cellCytoplasm
-
Tissue specificitySee included datasheet or contact our support service
-
Scientific contextThe 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia.
-
Bibliography1. Velasco M.E., et al. (1980) Cancer. 45:484. 2. Bonnin J.M., et al. (1984) Acta Neuropathology. 62:185. 3. Lee VM-Y., et al. (1984) J. Neurochem. 42:25-32 (1984). 4. Trojanowski JQ et al. (1986) J. Neurochem. 6(3): 650-660 (1986). 5. Schmidt ML et al; Lab Invest 56:282-294 (1987). 6. Kosik KS et al; Neuron 1:817-825 (1988). 7. Schmidt ML et al; Lab Invest 59:460-466 (1988). 8. Mokuna, K, et al: J Neurosci Res 23:396 (1989). 9. Molenaar, et al; Exp Neurology 108:1-9 (1990). 10. Tohyama T et al; Am J Pathol, 142:871-882 (1993). 11. Tohyama T et al; Am J Pathol 142:883-892 (1993). 12. Thilenius, A.R.B., et al; J. Immunol. 162(2): 643-650 (1999).
-
Released date28-Oct-2013
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
NCBI numberNP_001124491.1
-
Gene number2670
-
Protein numberP14136
-
Antibody s datasheetContact our support service
-
Representative figure link
-
Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-GFAP R416WT Monoclonal Antibody, Clone S206B-9 (SMC-442). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GFAP R416WT Monoclonal Antibody (SMC-442) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GFAP R416WT Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of GFAP protein using Mouse Anti-GFAP Monoclonal Antibody, Clone S206B-9 (SMC-442). Primary Antibody: Mouse Anti-GFAP Monoclonal Antibody (SMC-442) at 1:250. Mouse Anti-GFAP R416WT Antibody [S206B-9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-442) Mouse Anti-GFAP Antibody [S206B-9] used in Western Blot (WB) on Rat Brain Membrane (SMC-442)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilityIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Latin nameMus musculus
-
Gene target
-
Gene symbolGFAP, RNU6-9, PIRC47, PIRC38, PIRC43, PIRC45, PIRC39
-
Short nameMouse Anti- GFAP R416WT Monoclonal IgG1, Clone S206B-9
-
Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
-
Hostmouse
-
IsotypeIgG1, IgG1
-
LabelATTO 488
-
SpeciesHuman, Humans, Mouses
-
Alternative nameMouse Antibody toHuman GFAP R416WT monoclonal IgG1, clonality S206B-9
-
Alternative techniquemurine, antibodies
-
Clone nameS206B-9
-
Gene info
-
Identity
-
Gene
-
Long gene nameglial fibrillary acidic protein
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1989-12-07
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Intermediate filaments Type III
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameRNA, U6 small nuclear 9
-
Synonyms
-
Locus
-
Discovery year2008-06-10
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nuclear RNAs
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 47
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 38
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 43
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 45
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 39
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data