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Clone
FIH 162c
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Immunogen
Full length human FIH expressed in E.coli BL21 (DE3) cells
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Antibody s full description
Mouse Anti-Human FIH Monoclonal IgG1 Antibody, Clone: FIH 162c: Streptavidin
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
Factor inhibiting HIF1 (hypoxia-inducible factor) Antibody, DKFZp762F1811 Antibody, Factor inhibiting HIF-1 Antibody, Factor inhibiting HIF1 Antibody, FIH 1 Antibody, FIH-1 Antibody, FIH1 Antibody, FLJ20615 Antibody, FLJ22027 Antibody, HIF1AN Antibody, HIF1N_HUMAN Antibody, Hypoxia inducible factor 1 alpha inhibitor Antibody, Hypoxia inducible factor 1 alpha subunit inhibitor Antibody, Hypoxia inducible factor asparagine hydroxylase Antibody, Hypoxia-inducible factor 1-alpha inhibitor Antibody, Hypoxia-inducible factor asparagine hydroxylase Antibody, Peptide aspartate beta dioxygenase Antibody
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Raised in
Mouse
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Antibody s target
FIH
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Primary research fields
Cancer, Oxidative Stress, Cell Signaling, Epigenetics
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF
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Antibody s reactivity
Human, Mouse
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Antibody s dilutions
WB (1:100), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~45kDa.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-182 was sufficient for detection of FIH in 20 µg of Hela lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
FIH, Factor inhibiting HIF1 (hypoxia-inducible factor), is an asparaginyl hydroxylase. FIH in conjunction with VHL represses HIF-1 transcriptional activity by disrupting the interaction of HIF-1 with the transcriptional co-activators CBP/p300, and by recruiting histone deacetylases. FIH activity is inhibited during hypoxia (1-3). Recent studies show that low nuclear expression of FIH is a strong independent prognostic factor for a poor overall survival in clear cell renal cell carcinoma (4).
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Bibliography
1. Stolze I.P., et al. (2004) J Bio Chem. 42719-42725. 2. Soilleux E.J., et al. (2005) Histopathology 47:602-610. 3. Moon H., Han S., Park H., Choe J. (2010) Mol Cells. 29(5): 471-474. 4. Kroeze S.G., et al. (2010) Eur J Cancer. Epub.
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Released date
1-Sep-2010
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_060372.2
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Gene number
55662
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Protein number
Q9NWT6
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FIH Monoclonal Antibody, Clone fih162C (SMC-182). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (SMC-182) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-FIH Antibody. (C) Composite. Immunohistochemistry analysis using Mouse Anti-FIH Monoclonal Antibody, Clone fih162C (SMC-182). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (SMC-182) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All positive. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FIH Monoclonal Antibody, Clone fih162C (SMC-182). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (SMC-182) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-FIH Antibody. (C) Composite. Western Blot analysis of Human Cell lysates showing detection of FIH protein using Mouse Anti-FIH Monoclonal Antibody, Clone fih162c (SMC-182). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (SMC-182) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-FIH Antibody [fih162C] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-182) Mouse Anti-FIH Antibody [fih162C] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-182) Mouse Anti-FIH Antibody [fih162C] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-182) Mouse Anti-FIH Antibody [fih162c] used in Western Blot (WB) on Human Cell lysates (SMC-182)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
