Mouse Anti-Human Dnmt3L Monoclonal IgG1, Clone S117-9
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Catalog numberSMC-341D-A655
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Price:
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Size100 µg
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CloneS117-9
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ImmunogenFusion protein amino acids 1-387 of human DNMT3L
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Antibody s full descriptionMouse Anti-Human DNMT3L Monoclonal IgG1 Antibody, Clone: S117-9: ATTO 655
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameCytosine 5 methyltransferase 3 like protein antibody, DNA (cytosine 5 ) methyltransferase 3 like antibody, DNA (cytosine-5)-methyltransferase 3-like antibody, DNA cytosine 5 methyltransferase 3 like protein antibody, DNA methyltransferase 3 like protein antibody, DNM3L_HUMAN antibody, Dnmt 3L antibody, Dnmt3l antibody, Human cytosine 5 methyltransferase 3 like protein antibody, MGC1090 antibody
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Raised inMouse
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Antibody s targetDNMT3L
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Primary research fieldsCell Signaling, Epigenetics
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP, AM
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Antibody s reactivityHuman, Mouse
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~45kDa. No cross-reactivity against DNMT3a or DNMT3B.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-341 was sufficient for detection of DNMT3L in 10 µg of COS cell transiently transfected with Flag-tagged DNMT3L lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellNucleus
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextMethylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue (1-6). Studies show that DNMT3L regulates the activity of DNMT3A and DNMT3B and stimulates their catalytic activities (7). DNMT3L has specifically been linked to the process of carcinogenesis, thru its role in nuclear programming (8).
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Bibliography1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D. et al. (1992) Nucl. Acids Res. 20: 2287-2291. 3. Xie S. et al. (1999) Gene 236: 87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25(3):338-342. 7. Kareta MS., et al. (2006) J Biol Chem. 281(36): 25893-25902. 8. Gokul G., Ramakrishna G., and Khosla S. (2009) Epigenetics. 4(5): 322-329.
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Released date13-Apr-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_037501.2
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Gene number29947
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Protein numberQ9UJW3
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-Dnmt3L Monoclonal Antibody, Clone S117-9 (SMC-341). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Dnmt3L Monoclonal Antibody (SMC-341) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Lower layers of the epidermis have positive cell staining in the cytoplasm. Western Blot analysis of Human tissue lysate showing detection of Dnmt3L protein using Mouse Anti-Dnmt3L Monoclonal Antibody, Clone S117-9 (SMC-341). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Dnmt3L Monoclonal Antibody (SMC-341) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dnmt3L Monoclonal Antibody, Clone S117-9 (SMC-341). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Dnmt3L Monoclonal Antibody (SMC-341) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Staining in the nucleus, and accumulated around the nucleus. Clear nuclear demarcations. Mouse Anti-Dnmt3L Antibody [S117-9] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-341) Mouse Anti-Dnmt3L Antibody [S117-9] used in Western Blot (WB) on Human tissue lysate (SMC-341) Mouse Anti-Dnmt3L Antibody [S117-9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-341)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolRNU6-9, PIRC47, PIRC38, PIRC43, PIRC45, PIRC37
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Short nameMouse Anti- Dnmt3L Monoclonal IgG1, Clone S117-9
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 655
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman Dnmt3L monoclonal IgG1, clonality S117-9
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Alternative techniquemurine, antibodies
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Clone nameS117-9
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Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 9
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 47
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 38
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 43
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 45
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 37
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data