Mouse Anti-Human CENP-A Monoclonal IgG1, Clone 5A7-2E11
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Catalog numberSMC-202D-A700
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PricePlease ask
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Size100 µg
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Clone5A7-2E11
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ImmunogenSynthetic peptide corresponding to a portion of human CENP-A
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Antibody s full descriptionMouse Anti-Human CENPA Monoclonal IgG1 Antibody, Clone: 5A7-2E11: ATTO 700
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameCENP A Antibody, cenpa Antibody, Centromere auto antigen A Antibody, Histone H3 like centromeric protein A Antibody
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Raised inMouse
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Antibody s targetCENPA
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Primary research fieldsCell Signaling, Epigenetics
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BrandnameNone
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Antibodies applicationsWB, ICC/IF
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Antibody s reactivityHuman
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~18kDa. Recognizes Human CENP-A.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-202 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellNucleus , Chromosome , Centromere , Kinetochore
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextA replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.
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Bibliography1. Rieder C.L., et al. (1998) Trends Cell Biol. 8: 310-318. 2. Choo K.H. (2000) Trends Cell Biol. 10: 182-188. 3. Muro Y., et al. (2000) Clin. Exp. Immunol. 120: 218-223. 4. Howman E.V., et al. (2000) Proc. Natl. Acad. Sci. USA 97: 1148-1153.
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Released date23-Apr-2012
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_001035891.1
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Gene number1058
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Protein numberP49450
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11 (SMC-202). Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody (SMC-202) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X. Western Blot analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11 (SMC-202). Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody (SMC-202) at 1:1000. Mouse Anti-CENP-A Antibody [5A7-2E11] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Colon cancer cell line (HT-29) (SMC-202) Mouse Anti-CENP-A Antibody [5A7-2E11] used in Western Blot (WB) on Human U2OS cell lysate (SMC-202)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolCENPA, CENPU, HLA-A, CENPO, RASSF2, IGHV5-10-1, CIDEA, CENPQ, CENPT, RPA1
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Short nameMouse Anti- CENP-A Monoclonal IgG1, Clone 5A7-2E11
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 700
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman CENP-A monoclonal IgG1, clonality 5A7-2E11
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Alternative techniquemurine, antibodies
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Clone name5A7-2E11
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Gene info
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Identity
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Gene
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Long gene namecentromere protein A
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Synonyms gene name
- centromere protein A (17kD)
- centromere protein A, 17kDa
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-05-31
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Entrez gene record
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RefSeq identity
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Classification
- Constitutive centromere associated network
- H3 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecentromere protein U
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Synonyms gene
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Synonyms gene name
- MLF1 interacting protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Constitutive centromere associated network
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, A
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecentromere protein O
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-06-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Constitutive centromere associated network
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRas association domain family member 2
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Synonyms gene name
- Ras association (RalGDS/AF-6) domain family member 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-08-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ras association domain family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 5-10-1
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
- immunoglobulin heavy variable 5-A (provisional)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell death inducing DFFA like effector a
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Synonyms gene name
- cell death-inducing DFFA-like effector a
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecentromere protein Q
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Synonyms gene
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Synonyms gene name
- chromosome 6 open reading frame 139
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-06-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Constitutive centromere associated network
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecentromere protein T
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Synonyms gene
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Synonyms gene name
- chromosome 16 open reading frame 56
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-12-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Constitutive centromere associated network
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namereplication protein A1
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Synonyms gene name
- replication protein A1 (70kD)
- replication protein A1, 70kDa
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Nucleotide excision repair
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
