Mouse Anti-Human CD45 Monoclonal IgG1, Clone 12A9
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Catalog numberSMC-256D-FITC
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Price:
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Size100 µl
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Clone12A9
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ImmunogenPurified recombinant fragment of human CD45 expressed in E. Coli
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Antibody s full descriptionMouse Anti-Human CD45 Monoclonal IgG1 Antibody, Clone: 12A9: FITC
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameB220 Antibody, CD 45 Antibody, CD45R Antibody, GP180 Antibody, LCA Antibody, Protein tyrosine phosphatase receptor type c polypeptide Antibody, PTPRC_HUMAN Antibody, T200 glycoprotein Antibody
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Raised inMouse
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Antibody s targetCD45
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BrandnameNone
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Antibodies applicationsWB, IHC
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Antibody s reactivityHuman
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody buffer for storagePBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
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Antibody s specificityDetects endogenous CD45 proteins. (~147KD).
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA 1:1000 dilution of SMC-256 was sufficient for detection of CD45 in 10 µg of Human HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextCD45 is a 180-240 kD glycoprotein, also known as the leukocyte common antigen (LCA), T200 or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, broadly expressed amount hematopoietic cells. There are kmultiple isoforms of CD45 distributed throughout the immune system, and they arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell, as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
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Bibliography1. Barclay A., et al. (1997) The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge I.S., et al. (1993) Annu. Rev. Immunol. 12:85. 3. Kishihara K., et al. (1993) Cell. 74:143. 4. Pulido R., et al. (1988) J. Immunol. 140:3851.
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Released date06-Sep-2016
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_563578.2
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Gene number5788
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Protein numberP08575
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Antibody s datasheetContact our support service
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Representative figure legendWestern Blot analysis of Human HeLa cell lysates showing detection of ~147 kDa CD45 protein using Mouse Anti-CD45 Monoclonal Antibody, Clone 12A9 (SMC-256). Primary Antibody: Mouse Anti-CD45 Monoclonal Antibody (SMC-256) at 1:1000. Predicted/Observed Size: ~147 kDa. Mouse Anti-CD45 Antibody [12A9] used in Western Blot (WB) on Human HeLa cell lysates (SMC-256)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolPTPRCAP, PTPRC
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Short nameMouse Anti- CD45 Monoclonal IgG1, Clone 12A9
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman CD45 monoclonal IgG1, clonality 12A9
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Alternative techniquemurine, antibodies
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Clone name12A9
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Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C associated protein
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Synonyms
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Locus
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Discovery year1996-04-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data