Mouse Anti-Human Cav3.2 Monoclonal IgG1, Clone S55-10
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Catalog numberSMC-303D-A565
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PricePlease ask
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Size100 µg
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CloneS55-10
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ImmunogenFusion protein amino acids 1019-1293 (II-III loop) of human Cav3.2
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Antibody s full descriptionMouse Anti-Human Cav3.2 Monoclonal IgG1 Antibody, Clone: S55-10: ATTO 565
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameCav3.2 Antibody, CACNA1H Antibody, CACNA1HB Antibody, calcium channel Antibody, voltage-dependent Antibody, T type Antibody, alpha 1H subunit Antibody, calcium channel Antibody, voltage-dependent Antibody, T type Antibody, alpha 1Hb subunit Antibody, ECA6 Antibody, EIG6 Antibody, FLJ90484 Antibody, Low-voltage-activated calcium channel alpha1 3.2 subunit Antibody, low-voltage-activated calcium channel alpha13.2 subunit Antibody, voltage dependent t-type calcium channel alpha-1H subunit Antibody, voltage-dependent T-type calcium channel subunit alpha-1H Antibody, voltage-gated calcium channel alpha subunit Cav3.2 Antibody, voltage-gated calcium channel alpha subunit CavT.2 Antibody, Voltage-gated calcium channel subunit alpha Cav3.2 Antibody
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Raised inMouse
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Antibody s targetCav3.2
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Primary research fieldsNeuroscience, Ion Channels, Calcium Channels, Voltage-Gated Calcium Channels, Cancer, Cell Signaling
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP , AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~260kDa. No cross-reactivity against Cav1.3.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-303 was sufficient for detection of Cav3.2 in 10 µg of HEK cell lysate expressing Cav3.2 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextCaV3.2 is a protein which in humans is encoded by the CACNA1H gene. Studies suggest certain mutations in this gene lead to childhood absence epilepsy (1, 2). Studies also suggest that the up-regulations of CaV3.2 may participate in the progression of prostate cancer toward an androgen-independent stage (3).
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Bibliography1. Chen Y., et al. (2003) Ann. Neurol. 54(2): 239–43. 2. Khosravani H., et al. (2004) J Biol Chem. 279(11): 9681-9684. 3. Gackiere F., et al. (2008) J Biol Chem. 283(28): 19872.
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Released date6-Jul-2009
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_001005407.1
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Gene number8912
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Protein numberO95180
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody, Clone S55-10 (SMC-303). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody (SMC-303) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody, Clone S55-10 (SMC-303). Tissue: frozen brain section. Species: human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody (SMC-303) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Mouse Anti-CaV3.2 Calcium Channel Antibody [S55-10] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-303) Mouse Anti-CaV3.2 Calcium channel antibody [S55-10] used in Immunohistochemistry (IHC) on human frozen brain section (SMC-303)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolSNORD115-10, PIRC49, SNORD116-10, IGKV2OR2-10, IGHV3OR16-10, ERVK-10
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Short nameMouse Anti- Cav3 2 Monoclonal IgG1, Clone S55-10
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 565
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman Cav3.2 monoclonal IgG1, clonality S55-10
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Alternative techniquemurine, antibodies
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Clone nameS55-10
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-10
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 49
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-10
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2/OR2-10 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2/OR2-10
- immunoglobulin kappa variable 2/OR2-10 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa (IGK) orphons
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR16-10 (non-functional)
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Synonyms gene name
- immunoglobulin heavy variable 3/OR16-10
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group K member 10
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Synonyms gene name
- endogenous retrovirus group K, member 10
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Synonyms name
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GenBank acession
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Locus
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Discovery year2011-05-05
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Entrez gene record
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Pubmed identfication
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
