Mouse Anti-Human beta 4 Spectrin Monoclonal IgG1, Clone S393-2
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Catalog numberSMC-484D-BI
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PricePlease ask
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Size100 µg
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CloneS393-2
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ImmunogenFusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin
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Antibody s full descriptionMouse Anti-Human beta 4 Spectrin Monoclonal IgG1 Antibody, Clone: S393-2: Biotin
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameBetaIV-spectrin sigma1 Antibody, Non-erythrocytic spectrin beta chain 4 Antibody, Non-erythroidspectrin beta chain 3 Antibody, SPTBN4 Antibody, SPTBN3 Antibody, Spnb4 Antibody, KIAA1642 Antibody, EMBL BAB83243.1 Antibody, Ensembl ENSRNOP00000028393 Antibody
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Raised inMouse
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Antibody s targetbeta 4 Spectrin
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Primary research fieldsNeuroscience, Cell Signaling, Scaffolds
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~>200kDa. Does not cross react with other Beta-spectrins
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA 1:100 dilution of SMC-484 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm , Cytoskeleton , Cytoplasm , Cell Cortex
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextSpectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart (1). Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier (2).
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Bibliography1. Genes and mapped phenotypes. (n.d.). Retrieved March 12, 2015, from more 2. Yang Y., Lacas-Gervais S., Morest D.K., Solimena M., and Rasband M.N. (2004) J Neurosci. 24(33): 7230-7240.
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Released date10-Mar-2015
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_066022.2
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Gene number57731
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Protein numberQ9H254
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-beta 4 Spectrin Monoclonal Antibody, Clone S393-2 (SMC-484). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody (SMC-484) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) beta 4 Spectrin Antibody (D) Composite. Western Blot analysis of COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2 (SMC-484). Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody (SMC-484) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa. Mouse Anti-beta 4 Spectrin Antibody [S393-2] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-484) Mouse Anti-Beta-4-Spectrin Antibody [S393-2] used in Western Blot (WB) on COS-Beta-4-Spectrin-His (SMC-484)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolMIR7-2, MIR329-2, MIR512-2, MIR521-2, MIR1289-2, MIR509-2, KRTAP13-2, RNU6-2, SNORD116-2, SNORD114-2
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Short nameMouse Anti- beta 4 Spectrin Monoclonal IgG1, Clone S393-2
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelBiotin
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman b 4 Spectrin monoclonal IgG1, clonality S393-2
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Alternative techniquemurine, antibodies
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Clone nameS393-2
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Gene info
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Identity
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Gene
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Long gene namemicroRNA 7-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR7 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 329-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-11
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR329 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 512-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-15
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 521-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-15
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR515 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1289-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1289 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 509-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namekeratin associated protein 13-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-02-21
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Entrez gene record
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Pubmed identfication
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Classification
- Keratin associated proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 2
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-2
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data