Mouse Anti-Human beta 4 Spectrin Monoclonal IgG1, Clone S393-2

• Catalog number
  SMC-484D-APC
• Price
  Please ask
• Size
  100 µg

• Clone
  S393-2
• Immunogen
  Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin
• Antibody s full description
  Mouse Anti-Human beta 4 Spectrin Monoclonal IgG1 Antibody, Clone: S393-2: APC
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  BetaIV-spectrin sigma1 Antibody, Non-erythrocytic spectrin beta chain 4 Antibody, Non-erythroidspectrin beta chain 3 Antibody, SPTBN4 Antibody, SPTBN3 Antibody, Spnb4 Antibody, KIAA1642 Antibody, EMBL BAB83243.1 Antibody, Ensembl ENSRNOP00000028393 Antibody
• Raised in
  Mouse
• Antibody s target
  beta 4 Spectrin
• Primary research fields
  Neuroscience, Cell Signaling, Scaffolds
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.1% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~>200kDa. Does not cross react with other Beta-spectrins
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  A 1:100 dilution of SMC-484 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Cytoplasm , Cytoskeleton , Cytoplasm , Cell Cortex
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart (1). Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier (2).
• Bibliography
  1. Genes and mapped phenotypes. (n.d.). Retrieved March 12, 2015, from more 2. Yang Y., Lacas-Gervais S., Morest D.K., Solimena M., and Rasband M.N. (2004) J Neurosci. 24(33): 7230-7240.
• Released date
  10-Mar-2015
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_066022.2
• Gene number
  57731
• Protein number
  Q9H254
• Antibody s datasheet
  Contact our support service
• Representative figure link
  details | see here
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-beta 4 Spectrin Monoclonal Antibody, Clone S393-2 (SMC-484). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody (SMC-484) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) beta 4 Spectrin Antibody (D) Composite. Western Blot analysis of COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2 (SMC-484). Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody (SMC-484) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa. Mouse Anti-beta 4 Spectrin Antibody [S393-2] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-484) Mouse Anti-Beta-4-Spectrin Antibody [S393-2] used in Western Blot (WB) on COS-Beta-4-Spectrin-His (SMC-484)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Test
  StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Latin name
  Mus musculus

• Gene target
  beta   Spectrin   Monoclonal   IgG1   S393-2  
• Gene symbol
  MIR7-2, MIR329-2, MIR512-2, MIR521-2, MIR1289-2, MIR509-2, KRTAP13-2, RNU6-2, SNORD116-2, SNORD114-2
• Short name
  Mouse Anti- beta 4 Spectrin Monoclonal IgG1, Clone S393-2
• Technique
  anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG1, IgG1
• Label
  APC
• Species
  Human, Humans, Mouses
• Alternative name
  Mouse Antibody toHuman b 4 Spectrin monoclonal IgG1, clonality S393-2
• Alternative technique
  murine, antibodies
• Clone name
  S393-2

Gene info

• Identity
  HGNC:31639
• Gene
  MIR7-2
• Long gene name
  microRNA 7-2
• Synonyms gene
  • MIRN7-2
• Synonyms
  • hsa-mir-7-2
• Locus
  15q26.1
• Discovery year
  2004-04-23
• Entrez gene record
  407044
• RefSeq identity
  • NR_029606
• Classification
  • MicroRNA MIR7 family

Gene info

• Identity
  HGNC:32051
• Gene
  MIR329-2
• Long gene name
  microRNA 329-2
• Synonyms gene
  • MIRN329-2
• Synonyms
  • hsa-mir-329-2
• Locus
  14q32.31
• Discovery year
  2005-07-11
• Entrez gene record
  574409
• RefSeq identity
  • NR_029968
• Classification
  • MicroRNA MIR329 family

Gene info

• Identity
  HGNC:32091
• Gene
  MIR512-2
• Long gene name
  microRNA 512-2
• Synonyms gene
  • MIRN512-2
• Synonyms
  • hsa-mir-512-2
• Locus
  19q13.42
• Discovery year
  2005-07-15
• Entrez gene record
  574459
• RefSeq identity
  • NR_030181
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:32113
• Gene
  MIR521-2
• Long gene name
  microRNA 521-2
• Synonyms gene
  • MIRN521-2
• Synonyms
  • hsa-mir-521-2
• Locus
  19q13.42
• Discovery year
  2005-07-15
• Entrez gene record
  574481
• RefSeq identity
  • NR_030203
• Classification
  • MicroRNA MIR515 family

Gene info

• Identity
  HGNC:35282
• Gene
  MIR1289-2
• Long gene name
  microRNA 1289-2
• Synonyms gene
  • MIRN1289-2
• Synonyms
  • hsa-mir-1289-2
• Locus
  5q31.1
• Discovery year
  2008-11-03
• Entrez gene record
  100302134
• RefSeq identity
  • NR_031621
• Classification
  • MicroRNA MIR1289 family

Gene info

• Identity
  HGNC:33641
• Gene
  MIR509-2
• Long gene name
  microRNA 509-2
• Synonyms gene
  • MIRN509-2
• Synonyms
  • hsa-mir-509-2
• Locus
  Xq27.3
• Discovery year
  2007-10-24
• Entrez gene record
  100126301
• RefSeq identity
  • NR_030586
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:18923
• Gene
  KRTAP13-2
• Long gene name
  keratin associated protein 13-2
• Synonyms
  • KAP13-2
• GenBank acession
  • AP001708
• Locus
  21q22.11
• Discovery year
  2003-02-21
• Entrez gene record
  337959
• Pubmed identfication
  • 12359730
• Classification
  • Keratin associated proteins
• VEGA ID
  OTTHUMG00000057793

Gene info

• Identity
  HGNC:34270
• Gene
  RNU6-2
• Long gene name
  RNA, U6 small nuclear 2
• Synonyms
  • U6-2
• Locus
  19p13.3
• Discovery year
  2008-06-10
• Entrez gene record
  103625684
• Pubmed identfication
  • 12711679
• RefSeq identity
  • NR_125730
• Classification
  • Small nuclear RNAs

Gene info

• Identity
  HGNC:33068
• Gene
  SNORD116-2
• Long gene name
  small nucleolar RNA, C/D box 116-2
• Synonyms
  • HBII-85-2
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033414
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003317
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:32990
• Gene
  SNORD114-2
• Long gene name
  small nucleolar RNA, C/D box 114-2
• Synonyms
  • 14q(II-2)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767578
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003194.1
• Classification
  • Small nucleolar RNAs, C/D box

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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