Mouse Anti-Human AMIGO-1 Monoclonal IgG1, Clone S86-36
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Catalog numberSMC-438D
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PricePlease ask
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Size100 µg
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CloneS86-36
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ImmunogenFusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1
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Antibody s full descriptionMouse Anti-Human AMIGO1 Monoclonal IgG1 Antibody, Clone: S86-36: Unconjugated
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameAMIGO 1 Antibody, AMIGO1 Antibody, Adhesion molecule with Ig like domain 1 Antibody, Amphoterin-induced protein 1 Antibody, Alivin-2 Antibody, Alivin 2 Antibody, Ali2 Antibody, AMIGO Antibody, KIAA1163 Antibody, Amphoterin induced gene and ORF (Amigo) Antibody, Amphoterin induced protein 1 Antibody, MGC25558 Antibody, OTTHUMP00000013379 Antibody, RP23 89M15.6 Antibody
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Raised inMouse
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Antibody s targetAMIGO1
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Primary research fieldsNeuroscience, Cell Structure, Pre-Synaptic Markers
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~60-80kDa depending on maturity/glycosylation.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-438 was sufficient for detection of AMIGO-1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane , Cell Projection , Axon
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO1, AMIGO2 and AMIGO3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. They can form complexes with each other, but can also self-bind. AMIGO1, also designated Alivin2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO2 (Alivin1) is crucial for depolarization-dependent survival. Similar to AMIGO1 and AMIGO2, AMIGO3 (Alivin3) plays a role in hemophilic and/or heterophilic cell-cell interaction and signal transduction.
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Bibliography1. Kuja-Panula J., Kiiltomäki M., Yamashiro T., Rouhiainen A. and Rauvala H. (2003) J. Cell Biol. 160: 963-973. 2. Clark H.F., et al. (2003) Genome Res. 13: 2265-2270. 3. On, T., Sekino-Suzuki N., Kikkawa Y., Yonekawa H. and Kawashima S. (2003) J. Neurosci. 23: 5887-5896. 4. Chen Y., Aulia S., Li L. and Tang B.L. (2006) Brain Res. Brain Res. Rev. 51: 265-274.
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Released date5-Feb-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_065754.2
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Gene number57463
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Protein numberQ86WK6
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36 (SMC-438). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody (SMC-438) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) AMIGO-1 Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of 60-80 kDa AMIGO-1 protein using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36 (SMC-438). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody (SMC-438) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 60-80 kDa. Mouse Anti-AMIGO-1 Antibody [S86-36] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-438) Mouse Anti-AMIGO-1 Antibody [S86-36] used in Western Blot (WB) on Rat Brain Membrane (SMC-438)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolAMIGO1, SNORD115-36, IGKV2D-36, IGHV3-36, IGKV2-36, IGLV1-36, SNORA35, RNU6-36P, PDLIM1, THAP4
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Short nameMouse Anti- AMIGO-1 Monoclonal IgG1, Clone S86-36
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelUnconjugated
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman AMIGO-1 monoclonal IgG1, clonality S86-36
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Alternative techniquemurine, antibodies
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Clone nameS86-36
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Gene info
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Identity
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Gene
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Long gene nameadhesion molecule with Ig like domain 1
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Synonyms
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Synonyms name
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Locus
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Discovery year2005-06-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-36
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2D-36 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2D-36
- immunoglobulin kappa variable 2D-36 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-36 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-36
- immunoglobulin heavy variable 3-36 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2-36 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2-36
- immunoglobulin kappa variable 2-36 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 1-36
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, H/ACA box 35
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Synonyms
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Locus
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Discovery year2006-04-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, H/ACA box
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 36, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 36
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Locus
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Discovery year2008-06-10
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene namePDZ and LIM domain 1
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Synonyms gene
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Synonyms gene name
- PDZ and LIM domain 1 (elfin)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-01-21
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Entrez gene record
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Pubmed identfication
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Classification
- LIM domain containing
- PDZ domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTHAP domain containing 4
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-10-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- THAP domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data