Mouse Anti-GFP Polyclonal: ATTO 700
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Catalog numberSPC-1250D-A700
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Price:
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Size100 µl
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ImmunogenFull-length GFP protein.
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Antibody s targetGFP
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Antibody s full descriptionMouse Anti-GFP Polyclonal
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Primary research fieldsTags and Cell Markers, Fluorescent Proteins
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameGFP Antibody, Green fluorescent protein Antibody, yfp Antibody
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Verified applicationsWB, IHC, ICC/IF, IP
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Raised inMouse
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Antibody s reactivitySpecies Independent
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Antibody s recommended dilutions for useWB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityRecognizes GFP, EGFP and GFP, EGFP tag fusion proteins. It may also recognize RFP, YFP, CFP.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:10000 dilution of SPC-1250 was sufficient for detection of GFP in 10 µg of GFP Protein by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
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Antibody in cellContact our support service.
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Tissue specificityPhotocytes.
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Scientific contextGreen Fluorescent Protein (GFP) is a 27 kDa protein produced from the jellyfish Aequorea victoria, and is excited by and brightly fluoresces green (emission peak at a wavelength of 509nm) when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
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Bibliography1. Prendergast F.G. and Mann K.G. (1978) Biochemistry. 17 (17): 3448–53. 2. Tsien R.Y. (1998) Annual Review of Biochemistry. 67: 509–44.
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Released date6/Sep/2016
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NCBI numberRefer to NCBI
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Gene numberRefer to GenBank
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Protein numberRefer to Swiss-Prot
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure legendWestern blot analysis of Aequorea victoria GFP Protein showing detection of ~26.9kDa GFP protein using Mouse Anti-GFP Polyclonal Antibody (SPC-1250). Lane 1: 1:5,000 Dilution. Lane 2: 1:10,000 Dilution. Primary Antibody: Mouse Anti-GFP Polyclonal Antibody (SPC-1250). Predicted/Observed Size: ~26.9kDa. Mouse Anti-GFP Antibody used in Western blot (WB) on GFP Protein (SPC-1250)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Short nameMouse Anti-GFP Polyclonal: ATTO 700
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TechniquePolyclonal, anti-, Mouse, anti, antibody to, antibodies, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Hostmouse
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IsotypeIgG1
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LabelATTO 700
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toGFP polyclonal: ATTO 700
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Alternative techniquepolyclonals, murine, antibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data