Mouse Anti-Dityrosine (DT) Monoclonal IgG1, Clone 10A6
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Catalog numberSMC-521D-HRP
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Price:
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Size100 µg
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Clone10A6
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ImmunogenSynthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
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Antibody s full descriptionMouse Anti- Dityrosine Monoclonal IgG1 Antibody, Clone: 10A6: HRP
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameDityrosine Antibody, Dityrosine (DT) Antibody, DT Antibody, DY Antibody
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Raised inMouse
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Antibody s targetDityrosine
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Primary research fieldsCancer, Oxidative Stress, Protein Oxidation, Cell Signaling, Post Translational Modifications, Oxidation
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BrandnameMOLECULAR SIGNATURE®
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Antibodies applicationsWB, ELISA
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Antibody s reactivitySpecies Independent
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Antibody s dilutionsWB (1:1000); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
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Antibody s concentration1 mg/ml
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Antibody s specificitySpecific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA 1:1000 dilution of SMC-521 was sufficient for detection of dityrosine in 1 µg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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Antibody in cellSee included datasheet or contact our support service
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Tissue specificitySee included datasheet or contact our support service
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Released date23-Mar-2017
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberRefer to NCBI
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Gene numberRefer to GenBank
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Protein numberRefer to Swiss-Prot
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendWestern Blot analysis of Dityrosine-BSA Conjugate showing detection of 67 kDa Dityrosine-BSA using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6 (SMC-521). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: 3,5-Dibromotyrosine-BSA. Lane 4: Dityrosine-BSA. Lane 5: Bromotyrosine-BSA. Lane 6: 7-ketocholesterol-BSA. Load: 1 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody (SMC-521) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. Western Blot analysis of Human Cervical Cancer cell line (HeLa) showing detection of Dityrosine-BSA using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6 (SMC-521). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody (SMC-521) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Mouse Anti-Dityrosine Antibody [10A6] used in Western Blot (WB) on Dityrosine-BSA Conjugate (SMC-521) Mouse Anti-Dityrosine Antibody [10A6] used in Western Blot (WB) on Cervical Cancer cell line (HeLa) (SMC-521)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Short nameMouse Anti-Dityrosine (DT) Monoclonal IgG1, Clone 10A6
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelHRP
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toDityrosine (DT) monoclonal IgG1, clonality 10A6
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Alternative techniquemurine, antibodies
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Clone name10A6
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data