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Clone
6G3
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Immunogen
Synthetic 3,5-Dibromotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
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Antibody s full description
Mouse Anti- Dibromo-tyrosine Monoclonal IgG1 Antibody, Clone: 6G3: ATTO 488
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
3,5-Dibromo-tyrosine Antibody, 3,5-dibromotyrosine Antibody, Dibromotyrosine Antibody, Dibromo-tyrosine Antibody, DiBrY Antibody, Dibromotyrosine (DiBrY) Antibody, Dibromo-Tyrosine (DiBrY) Antibody, Dibromo-tyrosine [DiBrY] Antibody
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Raised in
Mouse
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Antibody s target
Dibromo-tyrosine
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Primary research fields
Cancer, Oxidative Stress, Protein Oxidation, Cell Signaling, Post Translational Modifications, Oxidation
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Brandname
MOLECULAR SIGNATURE®
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Antibodies applications
WB, ICC/IF, ELISA
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Antibody s reactivity
Species Independent
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Antibody s dilutions
WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Specific for 3,5-Dibromo-tyrosine. Does not cross-react with Nitrotyrosine.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
A 1:1000 dilution of SMC-506 was sufficient for detection of 3,5-Dibromotyrosine in 1 µg of 3, 5-Dibromotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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Antibody in cell
See included datasheet or contact our support service
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Tissue specificity
See included datasheet or contact our support service
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Released date
23-Mar-2017
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
Refer to Swiss-Prot
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3 (SMC-506). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody (SMC-506) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250: 1:1000 for 30-60 min at RT. (A,C,E,F) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain (C,D) Phalloidin Alex Fluor 633 F-Actin stain (E,F) Dibromo-tyrosine Antibody (G,F) Composite. Courtesy of: Dr. Robert Burke, University of Victoria. Western Blot analysis of 3, 5-Dibromotyrosine-BSA Conjugate showing detection of 67 kDa 3,5-Dibromotyrosine-BSA using Mouse Anti-3,5-Dibromotyrosine Monoclonal Antibody, Clone 6G3 (SMC-506). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: Nitrosylated-BSA. Lane 4: Dibromotyrosine-BSA. Load: 1 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-3,5-Dibromotyrosine Monoclonal Antibody (SMC-506) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. Western Blot analysis of Human Cervical Cancer cell line (HeLa) showing detection of 3-5-Dibromotyrosine-BSA using Mouse Anti-3-5-Dibromotyrosine Monoclonal Antibody, Clone 6G3 (SMC-506). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-3-5-Dibromotyrosine Monoclonal Antibody (SMC-506) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Mouse Anti-Dibromo-tyrosine Antibody [6G3] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Embryonic kidney cells (HEK293) (SMC-506) Mouse Anti-Dibromo-tyrosine Antibody [6G3] used in Western Blot (WB) on 3, 5-Dibromotyrosine-BSA Conjugate (SMC-506) Mouse Anti-Dibromo-tyrosine Antibody [6G3] used in Western Blot (WB) on Cervical Cancer cell line (HeLa) (SMC-506)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus