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Clone
4F8
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Immunogen
Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH).
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Antibody s full description
Mouse Anti- Nitrotryptophan Monoclonal IgG2b Antibody, Clone: 4F8: Alkaline Phosphatase
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
6-Nitrotryptophan Antibody, Nitrotryptophan (NW) Antibody, Nitrotryptophan Antibody, NW Antibody, 6-NO2-Trp Antibody
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Raised in
Mouse
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Antibody s target
Nitrotryptophan
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Primary research fields
Cell Signaling, Post Translational Modifications, Nitration
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Brandname
MOLECULAR SIGNATURE®
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Antibodies applications
WB, ELISA
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Antibody s reactivity
Species Independent
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Antibody s dilutions
WB (1:1000); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Specific for 6-Nitrotyrptophan modified proteins. Doss not cross-react with 7-Ketocholoesterol modified proteins.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
A 1:1000 dilution of SMC-519 was sufficient for detection of 6-Nitrotryptophan in 1 µg of 6-Nitrotyrptophan conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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Antibody in cell
See included datasheet or contact our support service
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Tissue specificity
See included datasheet or contact our support service
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Released date
23-Mar-2017
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
Refer to Swiss-Prot
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Antibody s datasheet
Contact our support service
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Representative figure legend
Western Blot analysis of 6-Nitrotryptophan-BSA Conjugate showing detection of 67 kDa 6-Nitrotryptophan-BSA using Mouse Anti-6-Nitrotryptophan Monoclonal Antibody, Clone 4F8 (SMC-519). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (1 µg). Lane 4: 6-Nitrotryptophan-BSA (0.5 µg). Lane 5: 6-Nitrotryptophan-BSA (1 µg). Lane 6: 7-Ketocholesterol-BSA (0.5 µg). Lane 7: 7-Ketocholesterol-BSA (1 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-6-Nitrotryptophan Monoclonal Antibody (SMC-519) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: Luminol for 1 min. Predicted/Observed Size: 67 kDa. Mouse Anti-Nitrotryptophan Antibody [4F8] used in Western Blot (WB) on 6-Nitrotryptophan-BSA Conjugate (SMC-519)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus