FITC Polystyrene Particles, 0.1%w/v, 3.0-3.9µm, 2mL

Description

FITC Polystyrene Particles, 0.1%w/v, 3.0-3.9µm are a used in cell biology assays such as cell counting, cell separation, flow cytometry (FC), calibration of flow cytometer machines and so on. Guaranteed to meet the size specification and produced from high quality materials, these microparticles will be a handy tool in your lab work.
Notes

For research use only.
Properties

This spherotech Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. spherotech FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody

Gene target

Polystyrene Particles w/v 0-3 9µm 2mL
Gene symbol

IGKV1OR2-0, SNORD112, H1-0, MIR1302-3, MIR509-3, MIR7-3, PIRC13, PIRC11
Short name

FITC Polystyrene Particles, 1%w/v, 3 -3 9µm, 2mL
Technique

FITC, Fluorescein
Label

FITC
Alternative name

fluorecein Polystyrene particles, 0.1%w/v, 3.0-3.9µm, 2mL
Alternative technique

fluorescine

Name

Fluorescent Antibody Technique
Concept

Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data