Custom DNA Ladder or Marker Production

At Gentaur, we understand that precision is everything in molecular biology. That’s why we’ve developed a cutting-edge approach to producing custom DNA ladders tailored to your exact specifications. Using advanced techniques, we create size standards that are not only highly accurate and reproducible but also optimized for performance across a wide range of applications.

Rather than relying on conventional fragment assembly, our method ensures consistency and clarity in every band — delivering the confidence you need in your gel electrophoresis results.

How We Make Your Custom DNA Ladder

At Gentaur, we don’t just mix DNA fragments — we use proven, state-of-the-art molecular biology methods to create high-quality, reliable DNA ladders designed just for your needs.

High-Fidelity PCR Amplification

We start by amplifying your specified DNA fragments using high-fidelity DNA polymerases. This ensures the fragments have exact base pair lengths with minimal errors — critical for accurate sizing in your gels.

Enzymatic Digestion & Fragment Assembly

For complex or unusual fragment patterns, we use restriction enzymes or site-specific nucleases to cut plasmid DNA at precise locations. This lets us generate DNA fragments of non-standard sizes or unique patterns tailored to your requirements.

Purification by Size-Exclusion Chromatography or Gel Extraction

Each fragment is purified to remove primer dimers, nonspecific products, and residual enzymes. We use size-exclusion chromatography or gel extraction techniques to isolate clean, size-specific DNA bands, ensuring your ladder has sharp, distinct bands on gels.

Labeling and Modification (Optional)

Need fluorescent tags, biotin, or other chemical labels on your ladder fragments? We perform post-PCR labeling or incorporate modified nucleotides during amplification to give you ladders compatible with fluorescence imaging, affinity capture, or other detection methods.

Quantification and Stoichiometric Balancing

Each fragment’s concentration is carefully measured using UV spectrophotometry and fluorometric assays. We then mix fragments at controlled ratios to produce ladders with optimized band intensities — so you get clear, easily interpretable patterns on your gels.

Quality Control & Validation

Before shipping, each batch undergoes:

1. Capillary electrophoresis for precise size confirmation
2. Spectrophotometric purity assessment to check DNA integrity
3. Gel electrophoresis testing under your requested conditions to verify ladder performance

Why This Matters for Your Research

1. Accurate fragment sizing: Your ladder matches the exact fragment sizes needed — no guesswork, no missing bands.
2. Consistent band intensity: Balanced concentrations give you clear visualization and reliable quantitation.
3. Compatibility with your detection method: Fluorescent or biotin-labeled ladders improve signal-to-noise ratio on specialized imaging platforms.
4. Reproducibility: Strict QC means every batch performs the same — so your data stays consistent over time.

What You Get

1. Custom DNA ladder with your chosen fragment sizes and band intensities
2. Optional chemical labels integrated for advanced detection
3. Ready-to-use or freeze-dried formats based on your preference
4. Full documentation including fragment map, concentration data, and QC reports
5. Expert support for integration into your protocols