Real Time PCR Test (96 ready to use lyophilized tubes - 50 µl Reaction Mix) for quantitative detection of HBV
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Catalog number
V5-96/3FRT
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Price
Please ask
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Size
96 tests
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Description
The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
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About
The term “freeze-dry” is a lay description for the process of lyophilization protein powder. It is the conversion of water from a frozen state to a gaseous state without going through a liquid state. Aliquoting with 25 mM HEPES-KOH, pH 7.2 and 1 mM DTT and 10% weight on volume glycerol is suitable for storage of the reconstituted powder. The freeze-dry process removes moisture from the cells of specimens while the specimens remain frozen. TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Group
PCR, polymerase chain reaction
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Short name
Real Time PCR Test (96 tubes - 50 µl ) for quantitative of HBV
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Technique
detection, Tubes, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. tube
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Alternative name
Quantitative real-time PCR test kit Test (96 ready to use lyophilized tubes - 50 µl Reaction Mix) to measure volumetric quantification on Hepatitis B virus
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Alternative technique
falcon, tests, dna-amplification
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Virus
hbv
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MeSH Data
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Name
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Concept
Scope note:
Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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