Rat Nuclear factor NF-kappa-B p105 subunit ELISA Kit
-
Catalog numberEK3996
-
PricePlease ask
-
Size96-wells plate (breakable strips)
-
-
Long nameELISA test kit for Rat Nuclear factor NF-kappa-B p105 subunit
-
Synonym nameRattus Nuclear factor NF-kappa-B p105 subunit ELISA Kit
-
General descriptionThis is a ready to use kit for performing ELISA tests for detection of Nuclear factor NF-kappa-B p105 subunit in samples from Rat
-
AntigenNuclear factor NF-kappa-B p105 subunit
-
Species reactivityRat
-
Sample typesOur Nuclear factor NF-kappa-B p105 subunit ELISA kit has been proven to work and successfully detect Nuclear factor NF-kappa-B p105 subunit in Rat
-
Kit contentsELISA test kit for Rat Nuclear factor NF-kappa-B p105 subunitAssay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
-
Detection wavelengt450 nm
-
Assay procedure1-4h
-
Summary of the prodecure1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
-
ApplicationsEnzyme Linked Immunosorbent Assay
-
Shipping storage shelf lifeThe Nuclear factor NF-kappa-B p105 subunit ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Rat Nuclear factor NF-kappa-B p105 subunit ELISA Kit is 6 months after delivery.
-
TipsDue to transportation and handling small amounts of the liquid components of the Rattus Nuclear factor NF-kappa-B p105 subunit ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
-
PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
-
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
-
DescriptionAplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
-
AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
Latin nameRattus norvegicus
-
Gene target
-
Gene symbolNFE4, NFE2, NEFH, NFKB1, INA, NFASC, NFATC1, NFATC2, NFYC, NFIL3
-
Short nameNuclear factor NF-kappa-B p105 subunit ELISA
-
TechniqueELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
-
HostRat, antigen assay ELISA kit 1 Kit (96 Wells)
-
SpeciesRat, Rats
-
Alternative nameRat Nuclear factor NF-kappa-B p105 functionnal sequence Enzyme-linked immunosorbent assay reagent
-
Alternative techniqueelisas, kits
-
Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
-
Gene info
-
Identity
-
Gene
-
Long gene namenuclear factor, erythroid 4
-
Synonyms
-
Locus
-
Discovery year2013-08-23
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namenuclear factor, erythroid 2
-
Synonyms gene name
- nuclear factor (erythroid-derived 2), 45kD
- nuclear factor (erythroid-derived 2), 45kDa
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1993-11-03
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Basic leucine zipper proteins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameneurofilament heavy chain
-
Synonyms gene name
- neurofilament, heavy polypeptide 200kDa
- neurofilament heavy polypeptide
- neurofilament heavy
-
Synonyms
-
Locus
-
Discovery year1988-08-31
-
Entrez gene record
-
RefSeq identity
-
Classification
- Intermediate filaments Type IV
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namenuclear factor kappa B subunit 1
-
Synonyms gene name
- nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1991-11-14
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- NF-kappa B complex subunits
- Ankyrin repeat domain containing
- IPT domain containing
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene nameinternexin neuronal intermediate filament protein alpha
-
Synonyms gene
-
Synonyms gene name
- internexin neuronal intermediate filament protein, alpha
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1999-03-24
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Intermediate filaments Type IV
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameneurofascin
-
Synonyms gene name
- neurofascin homolog (chicken)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2005-11-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Ig-like cell adhesion molecule family
- I-set domain containing
- Fibronectin type III domain containing
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namenuclear factor of activated T cells 1
-
Synonyms gene name
- nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
- nuclear factor of activated T-cells 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1994-11-16
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Nuclear factors of activated T-cells
- IPT domain containing
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namenuclear factor of activated T cells 2
-
Synonyms gene name
- nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
- nuclear factor of activated T-cells 2
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1994-11-16
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- IPT domain containing
- Nuclear factors of activated T-cells
- MicroRNA protein coding host genes
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namenuclear transcription factor Y subunit gamma
-
Synonyms gene name
- nuclear transcription factor Y, gamma
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1996-10-26
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- MicroRNA protein coding host genes
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namenuclear factor, interleukin 3 regulated
-
Synonyms gene
-
Synonyms gene name
- interleukin-3 binding protein 1
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1995-02-13
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Basic leucine zipper proteins
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
-
Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
-
Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data