RT-PCR Kit

Quantity:

Reagent properties

Molecular biology DNA amplification enzymes and reagents
Group

PCR, polymerase chain reaction, RT-PCR mixes
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

Gene target

Kit
Gene symbol

KIT, KITLG
Short name

RT-PCR Kit
Technique

RT-PCR, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
Alternative name

Reverse transcription PCR test kit reagent
Alternative technique

rtpcrkits, kits, dna-amplification
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Quantity:

Identity

HGNC:6342
Gene

KIT
Long gene name

KIT proto-oncogene, receptor tyrosine kinase
Synonyms gene
- PBT
Synonyms gene name
- piebald trait
- v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Synonyms
- CD117
- SCFR
- C-Kit
Synonyms name
- mast/stem cell growth factor receptor Kit
GenBank acession
- S67773
Locus

4q12
Discovery year

1986-01-01
Entrez gene record

3815
Pubmed identfication
- 9027509
RefSeq identity
- NM_000222
Classification
- Receptor tyrosine kinases
- CD molecules
- Immunoglobulin like domain containing
VEGA ID

OTTHUMG00000128713
Locus Specific Databases
- LRG_307

Name

Nucleic Acid Amplification Techniques
Concept

Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
Tree numbers
- E05.393.620
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data