Anti-KT3 FITC Polyclonal antibody

  • Catalog number
    18854F
  • Price
    Please ask
  • Size
    1mg
  • Clonality
    Polyclonal antibody
  • Long name
    Polyclonal antibody against KT3 FITC
  • Aplication
    You will receive the antybody in PBS(7,4 pH), for future delutions PBS or mediun similar to the one used for the assay are suitable.
  • Antigen
    KT3 FITC
  • Storage
    Could be stored for at least 12 month in frozen condition at -70C.It is recomendable to prepare aliquotes in order to avoid repetable freezing and thawing as they may denaturate the peptide chains/bonds in the antibodies and reduce/change the activity of the antibody. Prior to use briefly centrifuge the vial to collect its content on the bottom.
  • Product description
    Anti-KT3 FITC Polyclonal antibody can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by QED Biosciences they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This QED Biosciences Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. QED Biosciences FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
  • French translation
    anticorps
  • Gene target
    KT3  
  • Short name
    KT3 FITC pAb
  • Technique
    Polyclonal, Antibody, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Label
    FITC
  • Alternative name
    Antibody toKT3 fluorecein polyclonal (antibody to-)
  • Alternative technique
    polyclonals, antibodies, fluorescine
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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