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About recombinant protein
CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. The human CD40L gene codes for a 261 amino acid type II transmembrane protein, which contains a 22 amino acid cytoplasmic domain, a 24 amino acid transmembrane domain, and a 215 amino acid extracellular domain. The soluble form of CD40L is an 18 kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
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Other name of the recombinant
IGM, IMD3, TRAP, gp39, CD154, CD40L, HIGM1, T-BAM, TNFSF5, hCD40L, CD40 ligand, T-cell antigen Gp39, CD40-L
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Recombinant in
E. coli
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SEQUENCE
MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
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Activity
Assay 1: Determined by the stimulation of IL - 12 induction by human PBMCs.Assay 2: Determined by the dose - dependent stimulation of IL - 8 production by human PBMC. The expected ED50 for this effect is 5 - 10 ng/mL. Note: Results may vary with PBMC donors.
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Keep at
The lyophilized sCD40 Ligand recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted sCD40 Ligand is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing sCD40 Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
4557433
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Accession nr
NP_000065.1
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Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
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About
This product is for research use only.
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Protein s origin
Human
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Vial
Lyophilized
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NCBI ID
959
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Gene name
CD40 ligand
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Source
Homo sapiens
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Uniprot
P29965
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Description
FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
