PUMA Fragment Recombinant Protein

• Catalog number
  95-102
• Price
  Please ask
• Size
  0.05 mg

• About recombinant protein
  Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA- and PUMA- (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. This PUMA recombinant protein is recognized by several ProSci PUMA monoclonal antibodies, but not the two polyclonal antibodies (PSI # 3041 and 3043).
• Other name of the recombinant
  PUMA Antibody : JFY1, PUMA, JFY-1
• Recombinant in
  E. coli
• His tag on the recombinant protein
  Fusion Partner: His-tag and streptavidin II-tag at N-terminus
• SEQUENCE
  aa. 76 - 170
• Activity
  not available
• Use of recmbinant
  This recombinant protein can be used for WB, ELISA, MS. For research use only.
• Protein is used in
  E, WB, MS
• Suspention medium
  1X PBS containing 0.1% SDS and 0.02% NaN3
• Keep at
  Store in working aliquots at -70˚C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  56748610
• Accession nr
  Q9BXH1
• KDa of reombinant
  15 kDa (Calculated)
• Pureity
  ~95%
• About
  Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
• Vial
  Liquid
• NCBI ID
  27113
• Gene name
  BCL2 binding component 3
• Source
  Homo sapiens
• Uniprot
  Q9BXH1
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PUMA   Protein  
• Gene symbol
  BBC3
• Short name
  PUMA Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Isotype
  Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
• Alternative name
  PUMA Fragment Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:17868
• Gene
  BBC3
• Long gene name
  BCL2 binding component 3
• Synonyms
  • JFY1
  • PUMA
• Synonyms name
  • p53-upregulated modulator of apoptosis
• GenBank acession
  • AF332558
• Locus
  19q13.32
• Discovery year
  2002-07-22
• Entrez gene record
  27113
• Pubmed identfication
  • 11463392
  • 11572983
• RefSeq identity
  • NM_014417
• Classification
  • BCL2 homology region 3 (BH3) only
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000183489

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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