Mycoplasma Arginine Deiminase Recombinant Protein

• Catalog number
  40-582
• Price
  Please ask
• Size
  0.005 mg, 0.02 mg

• About recombinant protein
  Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G (1) and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis. Recombinant Mycoplasma Arginine Deiminase is a 46.4 kDa protein consisting of 409 amino acids.
• Other name of the recombinant
  MCRO_0627
• Recombinant in
  E. coli
• SEQUENCE
  MSVFDSKFKG IHVYSEIGEL ESVLVHEPGR EIDYITPARL DELLFSAILE SHDARKEHKQ FVAELKANDI NVVELIDLVA ETYDLASQEA KDKLIEEFLE DSEPVLSEEH KVVVRNFLKA KKTSRELVEI MMAGITKTDL GIEADHELIV DPMPNLYFTR DPFASVGNGV TIHYMRYKVR QRETLFSRFV FSNHPKLINT PWYYDPSLKL SIEGGDVFIY NNDTLVVGVS ERTDLQTVTL LAKNIVANKE CEFKRIVAIN VPKWTNLMHL DTWLTMLDKD KFLYSPIAND VFKFWDYDLV NGGAEPQPVE NGLPLEGLLQ SIINKKPVLI PIAGEGASQM EIERETHFDG TNYLAIRPGV VIGYSRNEKT NAALEAAGIK VLPFHGNQLS LGMGNARCMS MPLSRKDVKW
• Activity
  Biological activity was measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/mL. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
• Keep at
  The lyophilized Mycoplasma Arginine Deiminase recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Mycoplasma Arginine Deiminase stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Mycoplasma Arginine Deiminase recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  294155852
• Accession nr
  YP_003560236.1
• Pureity
  Greater than 97% by SDS-PAGE gel and HPLC analyses.
• About
  This product is for research use only.
• Protein s origin
  Mycoplasma crocodyli
• Vial
  Lyophilized
• NCBI ID
  8970640
• Gene name
  arginine deiminase (ADI) (Arginine dihydrolase) (AD)
• Source
  Mycoplasma crocodyli MP145
• Uniprot
  D5E643
• Pictures of recombinant
• Rec pdf
• Gene
  Mycoplasma is a genus of bacteria that lack a cell wall around their cell membrane. Without a cell wall, they are unaffected by many common antibiotics such as penicillin or other beta-lactam antibiotics that target cell wall synthesis. They can be parasitic or saprotrophic. Several species are pathogenic in humans, including M. pneumoniae, which is an important cause of atypical pneumonia and other respiratory disorders, and M. genitalium, which is believed to be involved in pelvic inflammatory diseases. Mycoplasma species are the smallest bacterial cells yet discovered, can survive without oxygen, and come in various shapes. For example, M. genitalium is flask-shaped (about 300 x 600 nm), while M. pneumoniae is more elongated (about 100 x 1000 nm). Hundreds of mycoplasmas infect animals
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Mycoplasma   Arginine   Deiminase   Protein  
• Gene symbol
  arcA
• Short name
  Arginine Deiminase Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Mycoplasma Arginine Deiminase Rec. Protein
• Alternative technique
  rec
• Disease
  mycoplasma

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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