MMP-3 Recombinant Protein
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Catalog number
40-525
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Price
Please ask
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Size
0.002 mg, 0.01 mg
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About recombinant protein
Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-3 degrades fibronectin, laminin, collagens III, IV, and X, and cartilage proteoglycans. Recombinant human MMP-3 is a 42.8 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain (378 amino acids).
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Other name of the recombinant
SL-1, STMY, STR1, CHDS6, MMP-3, STMY1, Stromelysin-1, Matrix metalloproteinase-3, SL-1
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Recombinant in
E. coli
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SEQUENCE
MRTFPGIPKW RKTHLTYRIV NYTPDLPKDA VDSAVEKALK VWEEVTPLTF SRLYEGEADI MISFAVREHG DFYPFDGPGN VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE IGHSLGLFHS ANTEALMYPL YHSLTDLTRF RLSQDDINGI QSLYGPPPDS PETPLVPTEP VPPEPGTPAN CDPALSFDAV STLRGEILIF KDRHFWRKSL RKLEPELHLI SSFWPSLPSG VDAAYEVTSK DLVFIFKGNQ FWAIRGNEVR AGYPRGIHTL GFPPTVRKID AAISDKEKNK TYFFVEDKYW RFDEKRNSME PGFPKQIAED FPGIDSKIDA VFEEFGFFYF FTGSSQLEFD PNAKKVTHTL KSNSWLNC
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Activity
MMP-3 activity was measured by its ability to cleave a chromogenic peptide MMP-3 substrate at room temperature. At a MMP-3 concentration of 2.5 μg/mL, 50% cleavage was achieved at an incubation time of approximately 75 minutes.
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Keep at
The lyophilized MMP-3 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted MMP-3 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing MMP-3 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
4505217
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Accession nr
NP_002413.1
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Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
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About
This product is for research use only.
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Protein s origin
Human
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Vial
Lyophilized
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NCBI ID
4314
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Gene name
matrix metallopeptidase 3 (stromelysin 1, progelatinase)
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Source
Homo sapiens
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Uniprot
P08254
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
MMP3
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Short name
MMP-3 Recombinant Protein
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
MMP-3 Rec. Protein
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Alternative technique
rec
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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