MIA Recombinant Protein

• Catalog number
  40-226
• Price
  Please ask
• Size
  0.005 mg, 0.02 mg

• About recombinant protein
  MIA is the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family; MIA, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. MIA is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis. Recombinant human MIA is a 12.2 kDa globular protein containing 108 amino acid residues including two intramolecular disulfide bonds.
• Other name of the recombinant
  CD-RAP, Melanoma-derived growth regulatory protein, Melanoma inhibitory activity protein
• Recombinant in
  E. coli
• SEQUENCE
  MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ
• Activity
  The ED50 was determined by the dose - dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4 - 6 μg/mL.
• Keep at
  The lyophilized MIA recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted MIA is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing MIA recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  321400133
• Accession nr
  NP_001189482.1
• Pureity
  Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  8190
• Gene name
  melanoma inhibitory activity
• Source
  Homo sapiens
• Uniprot
  Q16674
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  MIA   Protein  
• Gene symbol
  MIA
• Short name
  MIA Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  MIA Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:48352
• Gene
  MIA-RAB4B
• Long gene name
  MIA-RAB4B readthrough (NMD candidate)
• Locus
  19q13.2
• Discovery year
  2013-05-10
• Entrez gene record
  100529262
• VEGA ID
  OTTHUMG00000182782

Gene info

• Identity
  HGNC:7076
• Gene
  MIA
• Long gene name
  MIA SH3 domain containing
• Synonyms gene name
  • melanoma inhibitory activity
• Synonyms
  • CD-RAP
• GenBank acession
  • X75450
• Locus
  19q13.2
• Discovery year
  1999-12-14
• Entrez gene record
  8190
• Pubmed identfication
  • 7923218
  • 8661134
• Classification
  • MIA family
• VEGA ID
  OTTHUMG00000182693

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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