Lin28-TAT Recombinant Protein

• Catalog number
  40-562
• Price
  Please ask
• Size
  0.005 mg, 0.025 mg

• About recombinant protein
  Lin28 is a RNA-binding protein that belongs to a diverse family of structurally-related transcription factors. Lin28 is found abundantly in embryonic stem cells (ESCs), and to a lesser extent in placenta and testis. Lin28 has been shown to block let-7 microRNA processing and maturation, a necessary step in the differentiation of stem cells and certain cancer cell lines. Together with Sox2, Oct4, and Nanog, Lin28 can induce the reprogramming of primary human fibroblasts to a pluripotent state. Lin28 and other regulatory proteins can be introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing proteins into primary as well as transformed cells. Recombinant human Lin28-TAT is a 24.4 kDa protein containing 222 amino acid residues, including 13- residue C-terminal TAT peptide.
• Other name of the recombinant
  CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A, CSDD1, Protein lin-28 homolog A, Zinc finger CCHC domain-containing protein 1, Lin-28A
• Recombinant in
  E. coli
• SEQUENCE
  GPSVSNQQFA GGCAKAAEEA PEEAPEDAAR AADEPQLLHG AGICKWFNVR MGFGFLSMTA RAGVALDPPV DVFVHQSKLH MEGFRSLKEG EAVEFTFKKS AKGLESIRVT GPGGVFCIGS ERRPKGKSMQ KRRSKGDRCY NCGGLDHHAK ECKLPPQPKK CHFCQSISHM VASCPLKAQQ GP
• Activity
  Biological activity was measured by its ability to induce fluorescence in Lin28 reporter cells (293 cells transfected with fluorescent protein genes under Lin28 control). Optimum activity was achieved at 20 μg/mL after incubation for 72 hr. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
• Keep at
  The lyophilized Lin28-TAT recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Lin28-TAT stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Lin28-TAT recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  13375938
• Accession nr
  NP_078950.1
• Pureity
  Greater than 90% by SDS-PAGE gel and HPLC analyses.
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  79727
• Gene name
  lin-28 homolog A (C. elegans)
• Source
  Homo sapiens
• Uniprot
  Q9H9Z2
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Lin28-TAT   Protein  
• Gene symbol
  LIN28A
• Short name
  Lin28-TAT Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Lin28-TAT Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:15986
• Gene
  LIN28A
• Long gene name
  lin-28 homolog A
• Synonyms gene
  • LIN28
• Synonyms gene name
  • lin-28 homolog (C. elegans)
• Synonyms
  • LIN-28
  • FLJ12457
  • ZCCHC1
  • CSDD1
• GenBank acession
  • AF521099
• Locus
  1p36.11
• Discovery year
  2003-07-21
• Entrez gene record
  79727
• Pubmed identfication
  • 12798299
• RefSeq identity
  • NM_024674
• Classification
  • Zinc fingers CCHC-type
• VEGA ID
  OTTHUMG00000003550

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products