KLF4-TAT Recombinant Protein
-
Catalog number
40-524
-
Price
Please ask
-
Size
0.005 mg, 0.025 mg
-
-
About recombinant protein
KLF4 is a member of the Kruppel-like factor (KLF) family of zinc finger transcription factors. Members of this family have in common 3 contiguous C2H2-type zinc fingers at the carboxyl terminus that comprise the DNA-binding domain. KLF4 is highly expressed in skin and gut epithelial tissues, but is also found in various other cells and tissues, including vascular endothelial cells, lymphocytes, lung, and testis. It is an important regulator of the cell cycle, transcription, and cell differentiation. Together with Sox2, Oct4, and cMyc, KLF4 can induce the reprogramming of primary human fibroblasts to a pluripotent state. KLF4 and other transcription factors can be introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary as well as transformed cells. Recombinant human KLF4-TAT is a 51.7 kDa protein containing 483 amino acid residues, including 13- residue C-terminal TAT peptide.
-
Other name of the recombinant
EZF, GKLF, EZF, Krueppel-like factor 4, Epithelial zinc finger protein EZF
-
Recombinant in
HEK293 cells
-
SEQUENCE
MAVSDALLPS FSTFASGPAG REKTLRQAGA PNNRWREELS HMKRLPPVLP GRPYDLAAAT VATDLESGGA GAACGGSNLA PLPRRETEEF NDLLDLDFIL SNSLTHPPES VAATVSSSAS ASSSSSPSSS GPASAPSTCS FTYPIRAGND PGVAPGGTGG GLLYGRESAP PPTAPFNLAD INDVSPSGGF VAELLRPELD PVYIPPQQPQ PPGGGLMGKF VLKASLSAPG SEYGSPSVIS VSKGSPDGSH PVVVAPYNGG PPRTCPKIKQ EAVSSCTHLG AGPPLSNGHR PAAHDFPLGR QLPSRTTPTL GLEEVLSSRD CHPALPLPPG FHPHPGPNYP SFLPDQMQPQ VPPLHYQELM PPGSCMPEEP KPKRGRRSWP RKRTATHTCD YAGCGKTYTK SSHLKAHLRT HTGEKPYHCD WDGCGWKFAR SDELTRHYRK HTGHRPFQCQ KCDRAFSRSD HLALHMKRHF GGYGRKKRRQ RRR
-
Activity
Endotoxin level is less than 0.1 ng per ug (1EU/μg).
-
Keep at
The lyophilized KLF4-TAT recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted KLF4-TAT is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing KLF4-TAT recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
-
Rec PROTEIN GI
194248077
-
Accession nr
NP_004226.3
-
Pureity
Greater than 90% by SDS-PAGE gel and HPLC analyses.
-
About
This product is for research use only.
-
Protein s origin
Human
-
Vial
Lyophilized
-
NCBI ID
9314
-
Gene name
Kruppel-like factor 4 (gut)
-
Source
Homo sapiens
-
Uniprot
O43474
-
-
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
KLF4
-
Short name
KLF4-TAT Recombinant Protein
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Alternative name
KLF4-TAT Rec. Protein
-
Alternative technique
rec
-
Gene info
-
Identity
-
Gene
-
Long gene name
Kruppel like factor 4
-
Synonyms gene name
- Kruppel-like factor 4 (gut)
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1999-12-14
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Zinc fingers C2H2-type
- Kruppel like factors
-
VEGA ID
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products
