IL-4 Recombinant Protein
#
-
Catalog number40-619
-
Price:
-
Size0.02 mg
-
-
About recombinant proteinIL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant murine IL-4 is a 13.5 kDa globular protein containing 120 amino acid residues.
-
Other name of the recombinantIl-4, BSF-1, Il-4, Interleukin-4, B-cell IgG differentiation factor, IL-4
-
Recombinant inE. coli
-
His tag on the recombinant proteinno His tag
-
SEQUENCEMHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S
-
ActivityThe ED50 was determined by the dose-dependent stimulation of the proliferation of murine HT-2 cells is =2.0 ng/mL, corresponding to a specific activity of = 5 x 105 units/mg
-
Keep atThe lyophilized IL-4 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted IL-4 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing IL-4 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
-
Rec PROTEIN GI10946584
-
Accession nrNP_067258.1
-
PureityGreater than 98% by SDS-PAGE gel and HPLC analyses.
-
AboutThis product is for research use only.
-
Protein s originMurine
-
VialLyophilized
-
NCBI ID16189
-
Gene nameinterleukin 4
-
SourceMus musculus
-
UniprotP07750
-
Additional sourceRecombinants or rec. proteins
-
Grouprecombinants
-
Gene target
-
Gene symbolIl4
-
Short nameIL-4 Recombinant Protein
-
TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Alternative nameInterleukin-4 Rec. Protein
-
Alternative techniquerec
-
Gene info
-
Identity
-
Gene
-
Long gene nameinterleukin 4
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1988-08-10
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Interleukins
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data