IL-1 beta Recombinant Protein
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Catalog number
40-101
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Price
Please ask
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Size
0.002 mg, 0.01 mg
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About recombinant protein
IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Recombinant rat IL-1beta is a 17.3 kDa protein containing 153 amino acid residues.
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Other name of the recombinant
Interleukin-1 beta, IL-1 beta
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Recombinant in
E. coli
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SEQUENCE
MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS
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Activity
The ED50 was determined by the dose - dependent stimulation of murine D10S cells is < 0.1 ng/mL, corresponding to a specific activity of > 1 x 107 units/mg.
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Keep at
The lyophilized IL-1 beta recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted IL-1 beta is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing IL-1 beta recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
158186736
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Accession nr
NP_113700.2
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Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
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About
This product is for research use only.
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Protein s origin
Rat
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Vial
Lyophilized
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NCBI ID
24494
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Gene name
interleukin 1 beta
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Source
Rattus norvegicus
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Uniprot
Q63264
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Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
Il1b
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Short name
IL-1 beta Recombinant Protein
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
Interleukin-1 b Rec. Protein
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
interleukin 1 beta
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Synonyms
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GenBank acession
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Locus
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Discovery year
1989-03-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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