HGF Recombinant Protein
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Catalog number40-580
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PricePlease ask
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Size0.005 mg, 0.02 mg
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About recombinant proteinHGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Murine HGF is expressed as a linear 728 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains (α-chain and β-chain) held by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 233 amino acid residues.*Manufactured using (BTI-Tn-5B1-4) cells under license from the Boyce Thompson Institute for Plant Research, Inc.
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Other name of the recombinantSF, NK1, NK2, HGF/SF, SF/HGF, C230052L06Rik, Hepatocyte growth factor, Hepatopoietin-A, SF
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Recombinant in(BTI-Tn-5B1-4) High-5 Insect cells
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SEQUENCEAlpha chain: QKKRRNTLHE FKKSAKTTLT KEDPLLKIKT KKVNSADECA NRCIRNRGFT FTCKAFVFDK SRKRCYWYPF NSMSSGVKKG FGHEFDLYEN KDYIRNCIIG KGGSYKGTVS ITKSGIKCQP WNSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG PMDHTESGKT CQRWDQQTPH RHKFLPERYP DKGFDDNYCR NPDGKPRPWC YTLDPDTPWE YCAIKTCAHS AVNETDVPME TTECIQGQGE GYRGTSNTIW NGIPCQRWDS QYPHKHDITP ENFKCKDLRE NYCRNPDGAE SPWCFTTDPN IRVGYCSQIP KCDVSSGQDC YRGNGKNYMG NLSKTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNKNYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR Beta chain: VVNGIPTQTT VGWMVSLKYR NKHICGGSLI KESWVLTARQ CFPARNKDLK DYEAWLGIHD VHERGEEKRK QILNISQLVY GPEGSDLVLL KLARPAILDN FVSTIDLPSY GCTIPEKTTC SIYGWGYTGL INADGLLRVA HLYIMGNEKC SQHHQGKVTL NESELCAGAE KIGSGPCEGD YGGPLICEQH KMRMVLGVIV PGRGCAIPNR PGIFVRVAYY AKWIHKVILT YKL
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ActivityBiological activity was determined by the dose-dependent stimulation of the proliferation of mouse IMCD3 cells using a concentration range of 10-20 ng/mL. Endotoxin level is less than 0.1 ng per μg of HGF (1EU/μg).
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Keep atThe lyophilized HGF recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted HGF stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing HGF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI575501559
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Accession nrNP_001276387.1
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PureityGreater than 95% by SDS-PAGE gel and HPLC analyses.
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AboutThis product is for research use only.
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Protein s originMurine
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VialLyophilized
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NCBI ID15234
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Gene namehepatocyte growth factor
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SourceMus musculus
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UniprotQ08048
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolHgf
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Short nameHGF Recombinant Protein
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative namehepatocyte growth factor (hepapoietin A; scatter factor) Rec. Protein
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Alternative techniquerec
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Alternative to gene targethepatocyte growth factor (hepapoietin A; scatter factor), DFNB39 and F-TCF and HGFB and HPTA and SF, HGF and IDBG-24150 and ENSG00000019991 and 3082, protein heterodimerization activity, Extracellular, Hgf and IDBG-136449 and ENSMUSG00000028864 and 15234, HGF and IDBG-631194 and ENSBTAG00000017664 and 282879
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Gene info
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Identity
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Gene
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Long gene nameHGF activator
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-06-25
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 6
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Synonyms gene
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Synonyms gene name
- interleukin 6 (interferon, beta 2)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
- Interferons
- Interleukin 6 type cytokine family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSOS Ras/Rac guanine nucleotide exchange factor 1
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Synonyms gene
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Synonyms gene name
- gingival fibromatosis, hereditary, 1
- son of sevenless homolog 1 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-10-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Dbl family Rho GEFs
- Pleckstrin homology domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data