GDF-3 Recombinant Protein

• Catalog number
  40-592
• Price
  Please ask
• Size
  0.02 mg

• About recombinant protein
  GDF-3 is a member of the TGF-β superfamily of growth and differentiation factors, and is highly homologous to GDF-9. Unlike most TGF-β family members, GDF-3 and GDF-9 are not disulfide-linked dimers. GDF-3 is expressed in adult bone marrow, spleen, thymus, and adipose tissue. The expression of GDF-3 is upregulated in high-fat-fed wild-type FABP4/aP2 null mice and was associated with obesity, but not with the related hyperglycemia/hyperinsulinemia which characterizes Type 2 diabetes. Recombinant human GDF-3 is a 26.0 kDa non-disulfide-linked homodimer containing two 114 amino acid polypeptide chains.
• Other name of the recombinant
  KFS3, MCOP7, MCOPCB6GDF-3
• Recombinant in
  E. coli
• His tag on the recombinant protein
  no His tag
• SEQUENCE
  AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDE CGCG
• Activity
  Determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 100-150 ng/mL.
• Keep at
  The lyophilized GDF-3 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted GDF-3 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing GDF-3 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  10190670
• Accession nr
  NP_065685.1
• Pureity
  Greater than 98% by SDS-PAGE gel and HPLC analyses.
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  9573
• Gene name
  growth differentiation factor 3
• Source
  Homo sapiens
• Uniprot
  Q9NR23
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  GDF-3   Protein  
• Gene symbol
  GDF3
• Short name
  GDF-3 Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  GDF-3 Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:4218
• Gene
  GDF3
• Long gene name
  growth differentiation factor 3
• GenBank acession
  • AF263538
• Locus
  12p13.31
• Discovery year
  1999-04-23
• Entrez gene record
  9573
• Pubmed identfication
  • 9467948
• Classification
  • Transforming growth factor beta superfamily
• VEGA ID
  OTTHUMG00000168433

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products