G-CSF Recombinant Protein

• Catalog number
  40-555
• Price
  Please ask
• Size
  0.01 mg

• About recombinant protein
  G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues.
• Other name of the recombinant
  GE, NE, HLE, HNE, ELA2, SCN1, PMN-E, C17orf33, GCSF, Granulocyte colony-stimulating factor, Pluripoietin, G-CSF
• Recombinant in
  E. coli
• His tag on the recombinant protein
  no His tag
• SEQUENCE
  TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP
• Activity
  Biological activity was determined by its ability to stimulate the proliferation of mouse M-NFS-60 cells. The expected ED50 is ≤ 0.1 ng/mL, corresponding to a specific activity of ≥ 1 x 107 units/mg. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
• Keep at
  The lyophilized G-CSF recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted G-CSF stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing G-CSF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  4503549
• Accession nr
  NP_001963.1
• Pureity
  Greater than 98% by SDS-PAGE gel and HPLC analyses.
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  1991
• Gene name
  elastase, neutrophil expressed
• Source
  Homo sapiens
• Uniprot
  P09919-2
• Pictures of recombinant
• Rec pdf
• Gene
  Granulocyte-colony stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. Functionally, it is a cytokine and hormone, a type of colony-stimulating factor, and is produced by a number of different tissues. The pharmaceutical analogs of naturally occurring G-CSF are called filgrastim and lenograstim.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  G-CSF   Protein  
• Gene symbol
  ELANE
• Short name
  G-CSF Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  G-CSF Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:3309
• Gene
  ELANE
• Long gene name
  elastase, neutrophil expressed
• Synonyms gene
  • ELA2
• Synonyms gene name
  • elastase 2, neutrophil
• Synonyms
  • NE
  • HNE
  • HLE
  • PMN-E
• Synonyms name
  • neutrophil elastase
  • leukocyte elastase
  • medullasin
  • PMN Elastase
  • polymorphonuclear leukocyte elastase
• Locus
  19p13.3
• Discovery year
  1989-09-08
• Entrez gene record
  1991
• Pubmed identfication
  • 2902087
• RefSeq identity
  • NM_001972
• Classification
  • Granule associated serine proteases of immune defence
  • Complement system regulators and receptors
• VEGA ID
  OTTHUMG00000181839
• Locus Specific Databases
  • ELA2base: Mutation registry for Cyclic and congenital neutropenia
  • LRG_57

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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