Fetal Spleen Lysate
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Catalog numberXBL-10422
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Price:
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Size0.1 mg
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Lysates propertiesFetal human spleen tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 grades C. The fetal human spleen tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the spleen tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The spleen tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
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Lysate s originTissue
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State of tissueNormal
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Age of tissue lysateOther
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Species of the lysateHuman
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Cellular origibn of the lysateSpleen
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Disease of the lysateNormal
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Localisation of the biopsyFetal Human Spleen tissue
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Ug of lysate used by testFetal Spleen lysate is for use in Western blotting, 10 ug to 20 ug per lane is recommended for mini gel.
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Positive control inthe lysate is used as positive control in Western Blot
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PH and mediumHEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
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Keep atStore at 2-8 grades C for continuous use. For extended storage, freeze working aliquots at -70 grades C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
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CautionThis product is for research use only.
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Laboratory requirementsProducts are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. GENTAUR is not liable for damages or injuries resulting from receipt and/or use of GENTAUR materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. Also, for further information on the biosafety classification of human etiologic agents, please consult the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention's Office of Health and Safety (www.cdc.gov/od/ohs).
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Animal originHuman
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Dilution2 mg/mL or more concentrated
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Gene target
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Short nameFetal Spleen Lysate
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Alternative nameFetal Spleen Lysate
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Tissuelysate, spleen
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Viruslysate
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MeSH Data
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Name
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ConceptScope note: Methods for maintaining or growing CELLS in vitro.
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Tree numbers
- E01.370.225.500.223
- E05.200.500.265
- E05.242.223
- E05.481.500.249
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data