EndoG Fragment Recombinant Protein
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Catalog number95-103
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Price:
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Size0.05 mg
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About recombinant proteinThe fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribo-nuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1-3). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndoG also cleaves DNA in vitro (4).
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Other name of the recombinantEndoG Antibody : Endo G
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Recombinant inE. coli
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His tag on the recombinant proteinFusion Partner: His-tag and streptavidin II-tag at N-terminus
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SEQUENCEaa. 51 - 140
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Activitynot available
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Use of recmbinantThis recombinant protein can be used for WB, ELISA, MS. For research use only.
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Protein is used inE, WB, MS
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Suspention medium1X PBS containing 0.1% SDS and 0.02% NaN3
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Keep atStore in working aliquots at -70˚C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI24638471
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Accession nrQ14249
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KDa of reombinant15 kDa (Calculated)
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Pureity~95%
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AboutProducts are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
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VialLiquid
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NCBI ID2021
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Gene nameendonuclease G
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SourceHomo sapiens
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UniprotQ14249
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolENDOG
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Short nameEndoG Recombinant Protein
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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IsotypeAntibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
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Alternative nameendonuclease G Fragment Rec. Protein
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Alternative techniquerec
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Alternative to gene targetendonuclease G, ENDOG and IDBG-88694 and ENSG00000167136 and 2021, metal ion binding, multiple, Endog and IDBG-155637 and ENSMUSG00000015337 and 13804, ENDOG and IDBG-639600 and ENSBTAG00000012484 and 327707
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Gene info
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Identity
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Gene
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Long gene nameendonuclease G
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GenBank acession
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Locus
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Discovery year1994-09-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data