EndoG Fragment Recombinant Protein

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• Catalog number
  95-103
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• Size
  0.05 mg

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• About recombinant protein
  The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribo-nuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1-3). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndoG also cleaves DNA in vitro (4).
• Other name of the recombinant
  EndoG Antibody : Endo G
• Recombinant in
  E. coli
• His tag on the recombinant protein
  Fusion Partner: His-tag and streptavidin II-tag at N-terminus
• SEQUENCE
  aa. 51 - 140
• Activity
  not available
• Use of recmbinant
  This recombinant protein can be used for WB, ELISA, MS. For research use only.
• Protein is used in
  E, WB, MS
• Suspention medium
  1X PBS containing 0.1% SDS and 0.02% NaN3
• Keep at
  Store in working aliquots at -70˚C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  24638471
• Accession nr
  Q14249
• KDa of reombinant
  15 kDa (Calculated)
• Pureity
  ~95%
• About
  Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
• Vial
  Liquid
• NCBI ID
  2021
• Gene name
  endonuclease G
• Source
  Homo sapiens
• Uniprot
  Q14249
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

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• Gene target
  EndoG   Protein  
• Gene symbol
  ENDOG
• Short name
  EndoG Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Isotype
  Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
• Alternative name
  endonuclease G Fragment Rec. Protein
• Alternative technique
  rec
• Alternative to gene target
  endonuclease G, ENDOG and IDBG-88694 and ENSG00000167136 and 2021, metal ion binding, multiple, Endog and IDBG-155637 and ENSMUSG00000015337 and 13804, ENDOG and IDBG-639600 and ENSBTAG00000012484 and 327707

Gene info

• Identity
  HGNC:3346
• Gene
  ENDOG
• Long gene name
  endonuclease G
• GenBank acession
  • X79444
• Locus
  9q34.11
• Discovery year
  1994-09-29
• Entrez gene record
  2021
• Pubmed identfication
  • 7789991
• RefSeq identity
  • NM_004435
• VEGA ID
  OTTHUMG00000020763

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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