ApoE2 Recombinant Protein

• Catalog number
  40-140
• Price
  Please ask
• Size
  0.1 mg, 0.5 mg

• About recombinant protein
  ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. It’s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. It also binds with the LDL receptor (apo B/E). Defects in ApoE are a cause of hyperlipoproteinemia type III. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. Compared with E3 and E4, E2 exhibits the lowest receptor binding affinity. E2 allele carriers had significantly lower levels of total cholesterol, low-density lipoprotein cholesterol, and non-high-density lipoprotein cholesterol, as well as increased ApoE levels. Recombinant human ApoE2 is a 34.3 kDa protein containing 300 amino acid residues.
• Other name of the recombinant
  AD2, LPG, LDLCQ5, Apolipoprotein E, Apo-E
• Recombinant in
  E. coli
• SEQUENCE
  MKVEQAVETE PEPELRQQTE WQSGQRWELA LGRFWDYLRW VQTLSEQVQE ELLSSQVTQE LRALMDETMK ELKAYKSELE EQLTPVAEET RARLSKELQA AQARLGADME DVCGRLVQYR GEVQAMLGQS TEELRVRLAS HLRKLRKRLL RDADDLQKCL AVYQAGAREG AERGLSAIRE RLGPLVEQGR VRAATVGSLA GQPLQERAQA WGERLRARME EMGSRTRDRL DEVKEQVAEV RAKLEEQAQQ IRLQAEAFQA RLKSWFEPLV EDMQRQWAGL VEKVQAAVGT SAAPVPSDNH
• Keep at
  The lyophilized ApoE2 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted ApoE2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing ApoE2 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  4557325
• Accession nr
  NP_000032.1
• Pureity
  Greater than 90% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  348
• Gene name
  apolipoprotein E
• Source
  Homo sapiens
• Uniprot
  P02649
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  ApoE2   Protein  
• Gene symbol
  APOE
• Short name
  ApoE2 Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  ApoE2 Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:613
• Gene
  APOE
• Long gene name
  apolipoprotein E
• Synonyms gene
  • AD2
• Synonyms gene name
  • Alzheimer disease 2 (APOE*E4-associated, late onset)
• GenBank acession
  • K00396
• Locus
  19q13.32
• Discovery year
  2001-06-22
• Entrez gene record
  348
• Pubmed identfication
  • 10662539
• RefSeq identity
  • NM_000041
• Classification
  • Apolipoproteins
• VEGA ID
  OTTHUMG00000128901
• Locus Specific Databases
  • ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products