anti-WT1 Antibody

• Catalog number
  25-403
• Price
  Please ask
• Size
  50 ug

• Gene info
  WT1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms.This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
• Gene names
  WT1, GUD, WAGR, WIT-2, WT33, AWT1, NPHS4, EWS-WT1
• Antibodie s label
  unlabelled serum
• Purity
  Antibody is purified by peptide affinity chromatography method.
• Ab raised in
  Rabbit
• Type
  Polyclonal
• Antigen
  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human WT1.
• Immuno application
  ELISA, Western Blot
• Antibody advice
  WT1 antibody can be used for detection of WT1 by ELISA at 1:312500. WT1 antibody can be used for detection of WT1 by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Suspention pH azide
  Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
• Keep at
  For short periods of storage (days) store at 4 grades C. For longer periods of storage, store WT1 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
• Works with
  Human
• Omim nr
  65507817
• Ncbi nr
  NP_077742
• KDa
  56 kDa
• Vial with antibody
  Lyophilized
• Quantity per ml
  1 mg/ml
• NCBI GENE nr
  7490
• GENE symbol
  WT1
• Antigene name
  Wilms tumor 1
• Source
  Homo sapiens
• Protein nr
  P19544
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  WT1  
• Gene symbol
  WT1-AS, WT1
• Short name
  anti-WT1 Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toWT1 (antibody to-)
• Alternative technique
  antibodies
• Alternative to gene target
  Wilms tumor 1, AWT1 and EWS-WT1 and GUD and NPHS4 and WAGR and WIT-2 and WT33, WT1 and IDBG-37982 and ENSG00000184937 and 7490, C2H2 zinc finger domain binding, nuclei, Wt1 and IDBG-194872 and ENSMUSG00000016458 and 22431, WT1 and IDBG-636230 and ENSBTAG00000047268 and 782183

Gene info

• Identity
  HGNC:18135
• Gene
  WT1-AS
• Long gene name
  WT1 antisense RNA
• Synonyms gene
  • WIT1
• Synonyms gene name
  • Wilms tumor upstream neighbor 1
  • WT1 antisense RNA (non-protein coding)
• Synonyms
  • WIT-1
  • WT1AS
  • WT1-AS1
• Synonyms name
  • Wilms tumor associated protein
• GenBank acession
  • BC002734
• Locus
  11p13
• Discovery year
  2005-10-28
• Entrez gene record
  51352
• Pubmed identfication
  • 2173145
  • 8406502
  • 17210670
• RefSeq identity
  • NR_023920
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000039557

Gene info

• Identity
  HGNC:12796
• Gene
  WT1
• Long gene name
  WT1 transcription factor
• Synonyms gene
  • GUD
• Synonyms gene name
  • Wilms tumor 1
• Synonyms
  • WAGR
  • WIT-2
  • AWT1
  • NPHS4
  • WT-1
• Locus
  11p13
• Discovery year
  1989-04-13
• Entrez gene record
  7490
• Pubmed identfication
  • 14681303
  • 9810227
• RefSeq identity
  • NM_000378
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000039556
• Locus Specific Databases
  • LRG_525

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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