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Gene info
Neuraminidases or sialidases are exoglycosidases that catalyze the cleavage of a-glycosidically linked terminal N-acetyl neuraminic acid from sialylated glycoconjugates. They are widely spread in nature, occurring in viruses, bacteria, fungi, protozoa, birds and mammals. Together, the neuraminidases form a family of hydrolases that share a conserved active site and similar sequence motifs. Three types of neuraminidase are found in mammals and are defined as lysosomal, plasma membrane and cytosolic on the basis of their biochemical properties and subcellular distribution. Lysosomal N-acetyl-neuraminidase (NEU1) has significant primary structure characteristics of other mammalian and microbial sialidases with similar substrate specificity. However, unlike other members of this family, lysosomal neuraminidase requires the carboxypeptidase protective protein/cathepsin A (PPCA) for intracellular transport and lysosomal activation. The enzyme is only catalytically active when it is bound to PPCA and is a component of a high molecular weight, multi-protein complex containing PPCA, -galactosidase and N-acetylgalactosamine-6-sulfate sulfatase. The autosomal recessive genetic deficiency of NEU1 is associated with sialidosis, a neurodegenerative lysosomal storage disorder.
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Gene names
NEU1, Acetylneuraminyl hydrolase, G9 gene encoding sialidase, G9 sialidase, Exo-alpha-sialidase, Neu, Sialidase-1, Lysosomal sialidase, SIAL1, N-acetyl-alpha-neuraminidase 1, NANH
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Antibodie s label
unlabelled serum
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Purity
Sterile Filtration
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Sialidase 1 antibody was made against amino acids 21 - 31 of Sialidase 1 (Human). Bacterially expressed and purified fusion protein excluding the signal peptide (first 45 amino acids) and the first 6 N-terminal amino acids of the processed form of human lysosomal neuraminidase was used.
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Immuno application
Western Blot, immuno-histo-chemistry, immuno fluorescence
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Antibody advice
Sialidase 1 antibody can be used in ELISA, Western Blot starting at 1:1000 - 1:3000, and immunohistochemistry starting at 5 ug/mL.
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Suspention pH azide
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.
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Keep at
Store Sialidase 1 antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.
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Works with
Human
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Omim nr
17368612
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Ncbi nr
Q99519
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Vial with antibody
in solution
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NCBI GENE nr
4758
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GENE symbol
NEU1
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Antigene name
sialidase 1 (lysosomal sialidase)
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Source
Homo sapiens
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Protein nr
Q99519
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
